Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OH686_RS16115 Genome accession   NZ_CP126126
Coordinates   3447691..3449391 (-) Length   566 a.a.
NCBI ID   WP_289533915.1    Uniprot ID   -
Organism   Pseudomonas sp. SO81     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3442691..3454391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OH686_RS16080 (OH686_16470) - 3443341..3443790 (+) 450 WP_289539005.1 GNAT family N-acetyltransferase -
  OH686_RS16085 (OH686_16475) - 3443787..3444482 (+) 696 WP_289533905.1 energy-coupling factor ABC transporter permease -
  OH686_RS16090 (OH686_16480) - 3444576..3444791 (+) 216 WP_289533907.1 hypothetical protein -
  OH686_RS16095 (OH686_16485) yacG 3444792..3444986 (-) 195 WP_137970913.1 DNA gyrase inhibitor YacG -
  OH686_RS16100 (OH686_16490) coaE 3444983..3445594 (-) 612 WP_289533909.1 dephospho-CoA kinase -
  OH686_RS16105 (OH686_16495) pilD 3445591..3446463 (-) 873 WP_289533911.1 A24 family peptidase Machinery gene
  OH686_RS16110 (OH686_16500) pilC 3446467..3447684 (-) 1218 WP_289533913.1 type II secretion system F family protein Machinery gene
  OH686_RS16115 (OH686_16505) pilB 3447691..3449391 (-) 1701 WP_289533915.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OH686_RS16120 (OH686_16510) - 3449568..3450047 (-) 480 WP_289533917.1 pilin -
  OH686_RS16125 (OH686_16515) tfpZ 3450367..3451116 (+) 750 WP_289533919.1 TfpX/TfpZ family type IV pilin accessory protein -
  OH686_RS16130 (OH686_16520) nadC 3451305..3452153 (-) 849 WP_289533921.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62352.41 Da        Isoelectric Point: 5.4708

>NTDB_id=836777 OH686_RS16115 WP_289533915.1 3447691..3449391(-) (pilB) [Pseudomonas sp. SO81]
MNELPSLTGLAKQLVMTNLLDETSAQQAQLQAQRNKLPLVTYLAQNKLVKSRVLAELISDQFGIAMLDLSSIDKESMPRD
TVTEKLARQHRVLPLYKRGNKLFIATSDPSNHQAVTDVQFSTGLSTEALLVEDDKLGDAIEKFFDNAASGLADLGDVDLD
NVDIESVDDDKQDAGGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERVYRVRFRTDGILHEAARPPIQLAPRISARL
KVMASLDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEDNQKELYMNALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSTCKKPVDVPKEVLLKEGFPE
DKIGTFKIYGPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQTRRDGFNDLRTSGLLKAMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=836777 OH686_RS16115 WP_289533915.1 3447691..3449391(-) (pilB) [Pseudomonas sp. SO81]
ATGAACGAACTCCCCTCTCTGACCGGTCTCGCCAAGCAACTGGTCATGACCAACCTTCTGGACGAAACCTCGGCTCAGCA
GGCTCAGCTTCAGGCACAACGCAACAAACTCCCCCTCGTCACCTACCTGGCCCAGAACAAGCTGGTCAAGAGCCGGGTAC
TGGCCGAGCTGATCAGCGATCAATTCGGCATCGCCATGCTCGACCTGAGCAGCATCGACAAGGAGTCGATGCCGCGCGAC
ACGGTCACGGAAAAGCTCGCACGTCAGCATCGCGTGCTGCCACTCTACAAGCGTGGCAACAAGCTGTTCATCGCCACCTC
CGACCCTTCCAACCACCAGGCTGTCACCGATGTGCAGTTCAGCACCGGACTCAGCACCGAGGCCCTGCTGGTCGAGGACG
ACAAGCTCGGCGACGCCATCGAGAAATTCTTCGACAACGCCGCAAGCGGCCTGGCTGACTTGGGCGACGTGGACCTCGAC
AACGTCGATATCGAGAGCGTGGACGACGACAAGCAGGATGCCGGCGGCGGTGACGCCGACGATGCTCCGGTCGTGCGCTT
CGTCAACAAGATGCTCCTGGACGCCATCAAGGGCGGCTCTTCAGATCTCCATTTCGAACCTTACGAGCGCGTCTATCGCG
TGCGCTTTCGCACCGACGGCATTCTTCACGAAGCCGCCAGACCGCCGATACAGCTGGCCCCACGCATCTCCGCGCGCCTG
AAAGTCATGGCCAGCCTGGATATCTCCGAGCGCCGCAAGCCCCAGGACGGCCGGATCAAGATGAAAATTTCCAAGACCAA
GGCCATCGACTTCCGCGTCAACACCCTCCCCACGCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGACCCCACCAGCG
CGCAGATGGGAATCGACGCCCTGGGTTACGAGGACAATCAGAAAGAGCTGTACATGAATGCCCTGAAGCAACCCCAGGGC
ATGATTCTGGTAACCGGCCCTACCGGCTCCGGCAAGACGGTGTCCCTGTACACCGGCCTGAATATTCTCAATACCACCGA
TGTGAATATCTCCACCGCCGAAGACCCGGTAGAGATCAACCTCGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAGG
GCATGGACTTCTCCCAAGCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAAATTCGCGACCTG
GAAACAGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACATGGTCATGTCTACCCTGCACACCAACAGTGCAGCCGA
AACCCTGACCCGCCTGCGCAATATGGGAGTCCCGGCGTTCAACATCGCCACTTCGGTCAACCTGATTATTGCCCAGCGCC
TGGCACGCAAACTCTGCAGCACTTGCAAGAAACCAGTGGATGTTCCTAAAGAAGTCCTATTGAAAGAGGGCTTCCCAGAA
GACAAGATCGGCACCTTCAAGATCTACGGCCCCGTGGGATGCGAAAATTGCAAAGGTGGTTACAAGGGCCGGGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCACTGCAACGCATTATCATGGAGGAAGGCAACTCCATTGAAATCGCCGAGCAGA
CTCGCCGGGACGGCTTCAATGATCTGCGCACGTCCGGCCTGCTCAAGGCCATGCAAGGCGTTACCAGCCTTGAGGAAATC
AACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60

99.823

0.599
  pilB Acinetobacter baylyi ADP1

59.397

99.647

0.592
  pilB Legionella pneumophila strain ERS1305867

55.221

99.823

0.551
  pilB Vibrio cholerae strain A1552

49.643

98.94

0.491
  pilF Neisseria gonorrhoeae MS11

49.027

99.823

0.489
  pilB Vibrio campbellii strain DS40M4

48.155

100

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

50.657

94.17

0.477
  pilF Thermus thermophilus HB27

36.796

100

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

38.49

95.936

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.245

93.64

0.367