Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U8P18_RS18765 Genome accession   NZ_CP141061
Coordinates   3951716..3953101 (+) Length   461 a.a.
NCBI ID   WP_001025214.1    Uniprot ID   -
Organism   Escherichia coli strain KE55     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3946716..3958101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8P18_RS18740 (U8P18_18740) aroP 3947264..3948637 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  U8P18_RS18745 (U8P18_18745) ampE 3948680..3949534 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  U8P18_RS18750 (U8P18_18750) ampD 3949531..3950082 (-) 552 WP_000923734.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  U8P18_RS18755 (U8P18_18755) nadC 3950170..3951063 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  U8P18_RS18760 (U8P18_18760) pilA 3951266..3951706 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  U8P18_RS18765 (U8P18_18765) pilB 3951716..3953101 (+) 1386 WP_001025214.1 type II secretion system protein GspE Machinery gene
  U8P18_RS18770 (U8P18_18770) hofC 3953091..3954293 (+) 1203 WP_000157225.1 protein transport protein HofC -
  U8P18_RS18775 (U8P18_18775) guaC 3954328..3955371 (-) 1044 WP_322879751.1 GMP reductase -
  U8P18_RS18780 (U8P18_18780) - 3955527..3955571 (-) 45 WP_120795372.1 protein YacM -
  U8P18_RS18785 (U8P18_18785) coaE 3955596..3956216 (+) 621 WP_001269523.1 dephospho-CoA kinase -
  U8P18_RS18790 (U8P18_18790) zapD 3956216..3956959 (+) 744 WP_001194731.1 cell division protein ZapD -
  U8P18_RS18795 (U8P18_18795) yacG 3956969..3957166 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  U8P18_RS18800 (U8P18_18800) mutT 3957368..3957766 (-) 399 WP_000736024.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50639.36 Da        Isoelectric Point: 6.5396

>NTDB_id=835664 U8P18_RS18765 WP_001025214.1 3951716..3953101(+) (pilB) [Escherichia coli strain KE55]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSNELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLEMHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=835664 U8P18_RS18765 WP_001025214.1 3951716..3953101(+) (pilB) [Escherichia coli strain KE55]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGAATGAACTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATACGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGGAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACAGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATAAAAGCCGCACAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCTGCGAAACGCTGGTGCGTTTACAGCAAATGGGCGTTGCTCGCTGGATGCTCTCATCGGCACTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAATGCACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Haemophilus influenzae 86-028NP

40.127

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.478

83.948

0.382
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Acinetobacter baumannii D1279779

44.186

83.948

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36