Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   U8P18_RS18760 Genome accession   NZ_CP141061
Coordinates   3951266..3951706 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain KE55     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3946266..3956706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8P18_RS18740 (U8P18_18740) aroP 3947264..3948637 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  U8P18_RS18745 (U8P18_18745) ampE 3948680..3949534 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  U8P18_RS18750 (U8P18_18750) ampD 3949531..3950082 (-) 552 WP_000923734.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  U8P18_RS18755 (U8P18_18755) nadC 3950170..3951063 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  U8P18_RS18760 (U8P18_18760) pilA 3951266..3951706 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  U8P18_RS18765 (U8P18_18765) pilB 3951716..3953101 (+) 1386 WP_001025214.1 type II secretion system protein GspE Machinery gene
  U8P18_RS18770 (U8P18_18770) hofC 3953091..3954293 (+) 1203 WP_000157225.1 protein transport protein HofC -
  U8P18_RS18775 (U8P18_18775) guaC 3954328..3955371 (-) 1044 WP_322879751.1 GMP reductase -
  U8P18_RS18780 (U8P18_18780) - 3955527..3955571 (-) 45 WP_120795372.1 protein YacM -
  U8P18_RS18785 (U8P18_18785) coaE 3955596..3956216 (+) 621 WP_001269523.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=835663 U8P18_RS18760 WP_000360904.1 3951266..3951706(+) (pilA) [Escherichia coli strain KE55]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=835663 U8P18_RS18760 WP_000360904.1 3951266..3951706(+) (pilA) [Escherichia coli strain KE55]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363