Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QLH32_RS09200 Genome accession   NZ_CP125669
Coordinates   1937704..1939416 (+) Length   570 a.a.
NCBI ID   WP_283266010.1    Uniprot ID   -
Organism   Acinetobacter sp. KCTC 92772     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1932704..1944416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLH32_RS09165 (QLH32_09165) nusA 1933532..1935016 (-) 1485 WP_283266006.1 transcription termination factor NusA -
  QLH32_RS09170 (QLH32_09170) rimP 1935054..1935578 (-) 525 WP_283266007.1 ribosome maturation factor RimP -
  QLH32_RS09190 (QLH32_09190) secG 1936207..1936536 (-) 330 WP_283266008.1 preprotein translocase subunit SecG -
  QLH32_RS09195 (QLH32_09195) tpiA 1936549..1937340 (-) 792 WP_283266009.1 triose-phosphate isomerase -
  QLH32_RS09200 (QLH32_09200) pilB 1937704..1939416 (+) 1713 WP_283266010.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QLH32_RS09205 (QLH32_09205) pilC 1939440..1940672 (+) 1233 WP_283266011.1 type II secretion system F family protein Machinery gene
  QLH32_RS09210 (QLH32_09210) pilD 1940685..1941545 (+) 861 WP_283266012.1 A24 family peptidase Machinery gene
  QLH32_RS09215 (QLH32_09215) coaE 1941547..1942152 (+) 606 WP_283266013.1 dephospho-CoA kinase -
  QLH32_RS09220 (QLH32_09220) - 1942149..1943060 (-) 912 WP_283266014.1 DMT family transporter -
  QLH32_RS09225 (QLH32_09225) rlmB 1943091..1943840 (-) 750 WP_283266015.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  QLH32_RS09230 (QLH32_09230) - 1944023..1944349 (-) 327 WP_004680708.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63193.33 Da        Isoelectric Point: 5.1544

>NTDB_id=832336 QLH32_RS09200 WP_283266010.1 1937704..1939416(+) (pilB) [Acinetobacter sp. KCTC 92772]
MSGLHTSPKFSGFIRRLVEEGHLPASNMQNSIDAAKKAQQDIVAHLIEHQRLSPLTVAESISHEFGEPLFDLAVYDTALL
PKELTDNKLIQKHRVVPLVQRGQILYVATSNPTNIDSLDAIRFNTKLNIEPIIVEHNKLEKLIEQQFGDSGSFDFGDSED
FDLDVDVAEDSNKNDDEAPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCAQCKEVVEIPKQSLLELGF
TEQDLADPEFKIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVIQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=832336 QLH32_RS09200 WP_283266010.1 1937704..1939416(+) (pilB) [Acinetobacter sp. KCTC 92772]
ATGTCAGGATTACATACGTCTCCAAAATTTTCGGGGTTCATTCGGCGTTTAGTCGAAGAGGGGCATTTGCCTGCATCAAA
CATGCAGAATTCCATTGATGCGGCAAAAAAGGCCCAACAGGATATTGTGGCTCACCTGATTGAGCATCAGCGCCTCTCAC
CACTCACAGTTGCAGAAAGTATTTCCCATGAATTCGGTGAACCTCTGTTCGACTTGGCTGTATATGATACAGCCTTATTA
CCTAAGGAACTAACTGACAATAAGCTGATACAAAAACATCGGGTTGTACCTTTGGTGCAACGCGGCCAAATCCTATATGT
TGCGACCAGCAACCCGACCAATATTGATTCATTAGATGCCATTCGTTTTAATACCAAGCTCAATATTGAACCGATTATTG
TTGAACACAACAAACTTGAGAAACTGATTGAACAACAGTTTGGTGATAGTGGTAGTTTTGACTTCGGTGATAGTGAAGAT
TTTGACCTGGATGTGGATGTTGCTGAAGATTCGAATAAAAACGATGATGAGGCTCCTCAGGGAGATGAATCACCAATTGT
AAAATATATCAACAAACTGCTGATTGATGCGATTCGGATGGGTGCATCCGACCTACATTTTGAACCGTATGAAAAAACAT
ATCGAGTACGATACCGCGTGGATGGTGTATTACGACAGATTGCCACCCCCCCTTTACAAATGGCAAATCGTCTCGCATCC
CGTTTAAAAGTCATGTCACAGATGGACATTTCTGAAAAACGTATGCCACAGGATGGTCGTATTAAGCTAAAGCTATCCAA
AACCAAGGCCATTGACTTCCGTGTCAACTCTTTACCGACCCTGTTTGGTGAAAAACTGGTACTACGTATTCTCGATCCAT
CCAGTGCGATGTTGGGAATTGAAGCACTTGGCTACGAAGATGAACAAAAAGCCCTATTCCTCGAGGCATTGGATAAACCT
CAAGGCATGTTGCTTATTACAGGTCCAACAGGCTCAGGTAAAACGGTCTCCCTATATACTGGTCTCAATATACTCAATAC
TGAAGACACCAATATTTCCACAGCAGAAGATCCTGTCGAGATTAACCTGGAAGGGATTAACCAGGTCAATGTTAATCCTA
AAACGGGGCTAACTTTCTCTGCCGCACTAAAGGCATTCTTACGTCAAGATCCAGATGTGATCATGGTTGGGGAGATCCGT
GACCTTGAAACTGCTGAAATTGCCATTAAAGCTGCTCAGACAGGTCATATGGTGATGTCAACCCTCCATACCAATAGCGC
ACCTGAAACCCTAACCCGTTTACGTAATATGGGTGTACCATCCTTTAATATCGCAACTTCAGTCAATCTGGTTATTGCAC
AACGCTTGGCACGTCGTTTATGTGCTCAATGTAAGGAAGTTGTGGAAATTCCAAAACAGAGTCTGTTAGAACTCGGTTTT
ACTGAACAAGATCTCGCGGACCCAGAATTTAAGATATTTCAACCTGTAGGCTGTAGTGAATGCAGGGAAGGCTACAAGGG
CCGTGTCGGTATTTATGAAGTCATGAAAGTGACGCCAGAAATATCTAAAATCATCATGGAAGATGGTAATGCCCTACAAA
TTGCAGCAGCCTCAGAAAAGGCAGGATTCAACAATTTACGTCGCTCAGGTTTGAAAAAAGTGATACAGGGGATTACATCC
CTCCAGGAAGTCAACCGAGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.662

100

0.828
  pilB Acinetobacter baylyi ADP1

77.058

100

0.772
  pilB Legionella pneumophila strain ERS1305867

54.291

94.035

0.511
  pilF Neisseria gonorrhoeae MS11

49.378

98.772

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

49.281

97.544

0.481
  pilB Vibrio campbellii strain DS40M4

51.737

90.877

0.47
  pilB Vibrio cholerae strain A1552

51.158

90.877

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

34.839

100

0.379
  pilF Thermus thermophilus HB27

37.234

98.947

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.024

93.509

0.365