Detailed information NTG0831638

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QMK53_RS05330
• Genome accession: NZ_CP125379
• Coordinates: 1253293..1254405 (-)
• Length: 370 a.a.
• NCBI ID: WP_102355363.1
• Uniprot ID: -
• Organism: Pseudomonas sp. P8_139
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1248293..1259405

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QMK53_RS05310 (QMK53_05300)	-	1248892..1250283 (+)	1392	WP_320328098.1	GntP family permease	-
QMK53_RS05315 (QMK53_05305)	hbdH	1250291..1251064 (+)	774	WP_102355365.1	3-hydroxybutyrate dehydrogenase	-
QMK53_RS05320 (QMK53_05310)	-	1251223..1253178 (+)	1956	WP_102355364.1	acetoacetate--CoA ligase	-
QMK53_RS05330 (QMK53_05320)	pilU	1253293..1254405 (-)	1113	WP_102355363.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QMK53_RS05335 (QMK53_05325)	-	1254539..1257088 (+)	2550	WP_102355362.1	PAS domain-containing sensor histidine kinase	-
QMK53_RS05340 (QMK53_05330)	-	1257128..1257403 (+)	276	WP_003225447.1	peptidylprolyl isomerase	-
QMK53_RS05345 (QMK53_05335)	-	1257630..1258562 (-)	933	WP_102355361.1	sugar kinase	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 41167.16 Da
• Isoelectric Point: 6.8357

>NTDB_id=831638 QMK53_RS05330 WP_102355363.1 1253293..1254405(-) (pilU) [Pseudomonas sp. P8_139]
MEIDALLIRLSSQHGSDLFLSTGAPPSARIDGVLTPFSERPFKPGEVQAIANSLMDAEQRREFDRELEMNLAISRTGVGR
FRLNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKRSIINQREVGVDTRSFQAALKNTLRQAPDVVLIGEIRDRETMEHALTFADTGHLVLSTLHAHNANQALDRI
VNLFPEERRPQLLHALGNNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIADLIRRNELGELKGIMDKSAEVGMQTFDAAL
YALVVEGVISEDEALKHADSQNNLRLRLKLHAEAAPTPAPPASGEWGLMD

Nucleotide

• Length: 1113 bp

>NTDB_id=831638 QMK53_RS05330 WP_102355363.1 1253293..1254405(-) (pilU) [Pseudomonas sp. P8_139]
ATGGAAATCGATGCACTGTTGATCCGCCTGTCGAGCCAGCACGGCTCCGACCTGTTCCTCTCCACTGGCGCGCCGCCCAG
TGCGCGCATCGACGGGGTGCTGACGCCGTTCAGCGAGCGGCCGTTCAAACCGGGTGAGGTCCAGGCCATCGCCAACTCCC
TGATGGACGCCGAGCAGCGGCGCGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATTTCGCGCACCGGTGTCGGGCGT
TTTCGCCTGAACATCTTCAAACAACGCAACGACGTGTCAATCGTGATCCGCAACGTCAAGCTTGATATCCCGCGCTTCGA
AGACCTCAAGTTGCCTCCGGTGCTGCTGGAAACGGTGATGCTCAAGCAGGGGTTGATCCTGTTCGTCGGTGCCACCGACT
CCGGCAAATCCACCTCGTTGGCCGCGCTGATCGATCACCGCAACCGGCACAGCAGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGCGCTCGATCATCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGTTTCCAGGC
CGCGCTGAAAAACACCCTGCGCCAGGCTCCGGATGTGGTGCTGATCGGCGAAATCCGCGACCGCGAAACCATGGAACATG
CCCTGACGTTTGCCGACACCGGGCATCTGGTGCTGTCGACACTGCACGCGCACAACGCCAATCAGGCGCTGGACCGTATC
GTCAATCTGTTCCCCGAAGAACGCCGCCCGCAGTTGCTGCATGCGCTGGGCAACAATCTCAAGGCGTTTGTCTCGCAACG
GCTGGTGCGCACCCTCGACGGGCAGCGGCGGGCGGCGGTCGAGGTGATGTTGGGCACGCCGACCATCGCCGACCTGATCC
GCCGCAACGAGTTGGGCGAACTCAAGGGCATCATGGACAAATCGGCCGAAGTCGGGATGCAGACCTTCGATGCGGCGCTG
TACGCGCTGGTGGTGGAGGGTGTAATCAGCGAAGACGAGGCGCTCAAGCATGCCGACTCACAAAACAATCTGCGCCTGCG
CCTGAAGCTGCATGCCGAGGCGGCGCCGACGCCGGCACCACCGGCATCGGGCGAATGGGGCTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.775	95.946	0.535
pilU	Acinetobacter baylyi ADP1	52.809	96.216	0.508
pilU	Vibrio cholerae strain A1552	53.143	94.595	0.503
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.166	97.568	0.392
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	39.595	93.514	0.37
pilT	Vibrio cholerae strain A1552	39.595	93.514	0.37
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.973	0.368
pilT	Pseudomonas aeruginosa PAK	39.244	92.973	0.365