Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IFA22_RS20170 Genome accession   NZ_AP023398
Coordinates   4390391..4391500 (+) Length   369 a.a.
NCBI ID   WP_017411211.1    Uniprot ID   A0A1C2L453
Organism   Aeromonas hydrophila strain KAM330     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4385391..4396500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IFA22_RS20135 (KAM330_39450) - 4386009..4386320 (+) 312 WP_039213697.1 hypothetical protein -
  IFA22_RS20140 (KAM330_39460) - 4386375..4386794 (-) 420 WP_011707384.1 DUF4426 domain-containing protein -
  IFA22_RS20145 (KAM330_39470) yggU 4386813..4387112 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  IFA22_RS20150 (KAM330_39480) - 4387112..4387663 (-) 552 WP_016351969.1 YggT family protein -
  IFA22_RS20155 (KAM330_39490) proC 4387704..4388528 (-) 825 WP_077095139.1 pyrroline-5-carboxylate reductase -
  IFA22_RS20160 (KAM330_39500) - 4388587..4389288 (-) 702 WP_077095140.1 YggS family pyridoxal phosphate-dependent enzyme -
  IFA22_RS20165 (KAM330_39510) pilT 4389328..4390362 (+) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  IFA22_RS20170 (KAM330_39520) pilU 4390391..4391500 (+) 1110 WP_017411211.1 type IVa pilus ATPase TapU Machinery gene
  IFA22_RS20175 (KAM330_39530) yaaA 4391532..4392305 (+) 774 WP_077095141.1 peroxide stress protein YaaA -
  IFA22_RS20180 (KAM330_39540) srmB 4392482..4393705 (-) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  IFA22_RS20185 (KAM330_39550) - 4393834..4394544 (+) 711 WP_077095142.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  IFA22_RS20190 (KAM330_39560) brnQ 4394736..4396043 (+) 1308 WP_011707394.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41035.17 Da        Isoelectric Point: 6.5035

>NTDB_id=83113 IFA22_RS20170 WP_017411211.1 4390391..4391500(+) (pilU) [Aeromonas hydrophila strain KAM330]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=83113 IFA22_RS20170 WP_017411211.1 4390391..4391500(+) (pilU) [Aeromonas hydrophila strain KAM330]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGTAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTACAGGAGATCGCCCTGGCCAAACGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CTGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAATTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCATTCGACGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCGCCAGATGTGATCCTGATCGGCGAAATTCGCAGCCAGGAGACCATGGAGTTTG
CCCTCCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTAGACGGGCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGACATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAAGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTTACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2L453

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36

Multiple sequence alignment