Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   SPM78_RS23845 Genome accession   NZ_CP139424
Coordinates   5073443..5074066 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 136790     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5068443..5079066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPM78_RS23825 (SPM78_23825) aroB 5069038..5070144 (-) 1107 WP_023096238.1 3-dehydroquinate synthase -
  SPM78_RS23830 (SPM78_23830) aroK 5070194..5070712 (-) 519 WP_003095833.1 shikimate kinase AroK -
  SPM78_RS23835 (SPM78_23835) pilQ 5070724..5072868 (-) 2145 WP_023087871.1 type 4a pilus secretin PilQ Machinery gene
  SPM78_RS23840 (SPM78_23840) comL/pilP 5072922..5073446 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  SPM78_RS23845 (SPM78_23845) pilO 5073443..5074066 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  SPM78_RS23850 (SPM78_23850) pilN 5074063..5074659 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  SPM78_RS23855 (SPM78_23855) pilM 5074659..5075723 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  SPM78_RS23860 (SPM78_23860) - 5075908..5078379 (+) 2472 WP_003125627.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=830359 SPM78_RS23845 WP_003103268.1 5073443..5074066(-) (pilO) [Pseudomonas aeruginosa strain 136790]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=830359 SPM78_RS23845 WP_003103268.1 5073443..5074066(-) (pilO) [Pseudomonas aeruginosa strain 136790]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAACCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401