Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QLQ87_RS12825 Genome accession   NZ_CP124841
Coordinates   2787858..2789564 (+) Length   568 a.a.
NCBI ID   WP_282683320.1    Uniprot ID   A0AAX3VMM2
Organism   Aeromonas salmonicida strain 57     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2782858..2794564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLQ87_RS12800 (QLQ87_12800) pdhR 2783989..2784753 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QLQ87_RS12805 (QLQ87_12805) ampD 2785030..2785602 (-) 573 WP_212539623.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QLQ87_RS12810 (QLQ87_12810) - 2785752..2786222 (+) 471 WP_265466616.1 retropepsin-like aspartic protease -
  QLQ87_RS12815 (QLQ87_12815) nadC 2786227..2787084 (+) 858 WP_282683318.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QLQ87_RS12820 (QLQ87_12820) tapA 2787420..2787851 (+) 432 WP_282683319.1 type IVa pilus major pilin TapA -
  QLQ87_RS12825 (QLQ87_12825) pilB 2787858..2789564 (+) 1707 WP_282683320.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QLQ87_RS12830 (QLQ87_12830) pilC 2789697..2790938 (+) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  QLQ87_RS12835 (QLQ87_12835) pilD 2791002..2791877 (+) 876 WP_282683321.1 A24 family peptidase Machinery gene
  QLQ87_RS12840 (QLQ87_12840) coaE 2791897..2792511 (+) 615 WP_282683322.1 dephospho-CoA kinase -
  QLQ87_RS12845 (QLQ87_12845) zapD 2792549..2793271 (+) 723 WP_021140336.1 cell division protein ZapD -
  QLQ87_RS12850 (QLQ87_12850) yacG 2793281..2793475 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  QLQ87_RS12855 (QLQ87_12855) mutT 2793553..2793960 (-) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62633.06 Da        Isoelectric Point: 5.3215

>NTDB_id=828315 QLQ87_RS12825 WP_282683320.1 2787858..2789564(+) (pilB) [Aeromonas salmonicida strain 57]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRDASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKVQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=828315 QLQ87_RS12825 WP_282683320.1 2787858..2789564(+) (pilB) [Aeromonas salmonicida strain 57]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAGGCCAAGGCTCAGCGCAAACCATTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTACTGGATCTTGCCGCTTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGACACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCTGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCAGATGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGACGCATCCGACCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGGATCGATGGCATACTGCACGAGATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCCGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGACTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGTACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATATTGGTGACAGGCCCGACCGGCTCTGGCAAAACAGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTCAACATCTCTACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCTTCGGTAACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCCGAGGCTGAACTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAACCGGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CCATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Acinetobacter baumannii D1279779

55.203

99.824

0.551
  pilB Legionella pneumophila strain ERS1305867

54.417

99.648

0.542
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.418

99.296

0.511
  pilF Neisseria gonorrhoeae MS11

46.277

99.296

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377