Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QK281_RS02400 Genome accession   NZ_CP124746
Coordinates   499178..500884 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain NN-MR659     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494178..505884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QK281_RS02370 (QK281_02370) mutT 494854..495267 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  QK281_RS02375 (QK281_02375) yacG 495328..495522 (-) 195 WP_024945432.1 DNA gyrase inhibitor YacG -
  QK281_RS02380 (QK281_02380) zapD 495532..496254 (-) 723 WP_282467143.1 cell division protein ZapD -
  QK281_RS02385 (QK281_02385) coaE 496292..496906 (-) 615 WP_043126913.1 dephospho-CoA kinase -
  QK281_RS02390 (QK281_02390) pilD 496926..497798 (-) 873 WP_282467144.1 A24 family peptidase Machinery gene
  QK281_RS02395 (QK281_02395) pilC 497821..499062 (-) 1242 WP_043126919.1 type II secretion system F family protein Machinery gene
  QK281_RS02400 (QK281_02400) pilB 499178..500884 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QK281_RS02405 (QK281_02405) tapA 500888..501316 (-) 429 WP_044800922.1 type IVa pilus major pilin TapA -
  QK281_RS02410 (QK281_02410) nadC 501639..502496 (-) 858 WP_282467145.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QK281_RS02415 (QK281_02415) - 502502..502972 (-) 471 WP_024944772.1 retropepsin-like aspartic protease -
  QK281_RS02420 (QK281_02420) ampD 503120..503692 (+) 573 WP_124251318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QK281_RS02425 (QK281_02425) pdhR 503968..504732 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=827892 QK281_RS02400 WP_029302067.1 499178..500884(-) (pilB) [Aeromonas hydrophila strain NN-MR659]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=827892 QK281_RS02400 WP_029302067.1 499178..500884(-) (pilB) [Aeromonas hydrophila strain NN-MR659]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAAGCATTGG
CGGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTAGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACATCACGTCCTGCCCATCTATACCCAAGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAGCGATCAAGATGCTCTCGGCATGGAGGACATTGATGAATCCGAGATC
TCCGAACTGGAAGTGTCTGACGAGGGATCGAGGCTCGACGAGAGCGTCAATACGGCCGACGACGACGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAAACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCGACTCCACCTGTCAATTTGGCTAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGATTGCCCCAAGATGGTCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTACCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGGCAAAAAGCGCAATACTTGCGTGCCCTCTCGAAGCCTCAG
GGCATGATCTTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGTGCCCTGCGTTCTTTCTTGCGACAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACTTGGTGCTATCAACCCTGCACACCAACTCGGCGGC
GGAGACGCTGACCCGGATGATGAACATGGGGGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCAGGTTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCAGGTGGGTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAAGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCTTGGGGGTGACCAGTCTGGCA
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373