Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QIF44_RS15555 Genome accession   NZ_CP124546
Coordinates   3448578..3449720 (-) Length   380 a.a.
NCBI ID   WP_025874039.1    Uniprot ID   -
Organism   Stenotrophomonas indicatrix strain MGMM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3443578..3454720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QIF44_RS15530 (QIF44_15530) - 3443992..3444699 (-) 708 WP_025874035.1 YitT family protein -
  QIF44_RS15535 (QIF44_15535) - 3445176..3445388 (-) 213 WP_032977298.1 DUF4287 domain-containing protein -
  QIF44_RS15540 (QIF44_15540) - 3445783..3446682 (+) 900 WP_025874037.1 LysR family transcriptional regulator -
  QIF44_RS15545 (QIF44_15545) - 3446740..3447723 (-) 984 WP_032977300.1 aldo/keto reductase -
  QIF44_RS15550 (QIF44_15550) - 3447866..3448534 (-) 669 WP_152543319.1 PIN domain-containing protein -
  QIF44_RS15555 (QIF44_15555) pilU 3448578..3449720 (-) 1143 WP_025874039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QIF44_RS15560 (QIF44_15560) pilT 3449821..3450858 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  QIF44_RS15565 (QIF44_15565) - 3450946..3451623 (+) 678 WP_032977304.1 YggS family pyridoxal phosphate-dependent enzyme -
  QIF44_RS15570 (QIF44_15570) proC 3451640..3452461 (+) 822 WP_032977306.1 pyrroline-5-carboxylate reductase -
  QIF44_RS15575 (QIF44_15575) - 3452516..3453391 (-) 876 WP_282279639.1 LysR family transcriptional regulator -
  QIF44_RS15580 (QIF44_15580) - 3453490..3454092 (+) 603 WP_025874043.1 short chain dehydrogenase -
  QIF44_RS15585 (QIF44_15585) soxR 3454130..3454564 (-) 435 WP_032951587.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42365.71 Da        Isoelectric Point: 6.7684

>NTDB_id=826397 QIF44_RS15555 WP_025874039.1 3448578..3449720(-) (pilU) [Stenotrophomonas indicatrix strain MGMM10]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKELMKESVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=826397 QIF44_RS15555 WP_025874039.1 3448578..3449720(-) (pilU) [Stenotrophomonas indicatrix strain MGMM10]
GTGAACACCACCGCGACCACCATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACTGCCGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATTTCGCCGATCACCCAGACGCCACTGACGCCGCAGCAGA
GCCGCGACCTGGTGCTGAACGTGATGACGCCAGCGCAGCGCGAGGAATTCGAAAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGCGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCCACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGCGCTACCGGTACCGGTAAATCAACCTCGCTGGCAGCGATGATCGGTTACCGCAACCAGAATTCGACCGGG
CACATCATCACCATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
CGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATCATGATCGGCGAGGTGCGTA
CCCGCGAGGGCATGGACCACGCCATCGCCTTCGCTGAAACCGGTCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCC
AACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGATCGCCGCAACCAGCTGTTGATGGATCTGTCACTGAACCT
GAAGGGCGTGGTCGCGCAGCAGTTGATTCCTTCGCCGGATGGCCGCTCGCGCAAGGTGGCGATGGAGATCCTGCTGGGCA
CGCCGCTGGTGCAGGACTACATCCGCGACGGTGAGATCCACAAGCTGAAGGAGCTGATGAAGGAATCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGAGGACGCACTGCGCTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGATGCCCGCACCCTGTCGCAGGGGTTGGATGGCG
TGGAGATTTCAGAGGTCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

96.579

0.653
  pilU Acinetobacter baylyi ADP1

64.146

93.947

0.603
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.817

93.421

0.4
  pilT Pseudomonas aeruginosa PAK

40.896

88.158

0.361
  pilT Acinetobacter nosocomialis M2

39.826

90.526

0.361
  pilT Acinetobacter baumannii D1279779

39.826

90.526

0.361
  pilT Acinetobacter baumannii strain A118

39.826

90.526

0.361