Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SA976_RS20515 Genome accession   NZ_CP137765
Coordinates   4182406..4183791 (+) Length   461 a.a.
NCBI ID   WP_001025155.1    Uniprot ID   -
Organism   Escherichia coli strain GCEC19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4177406..4188791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA976_RS20490 (SA976_20490) aroP 4177954..4179327 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  SA976_RS20495 (SA976_20495) ampE 4179370..4180224 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  SA976_RS20500 (SA976_20500) ampD 4180221..4180772 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SA976_RS20505 (SA976_20505) nadC 4180860..4181753 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SA976_RS20510 (SA976_20510) pilA 4181956..4182396 (+) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  SA976_RS20515 (SA976_20515) pilB 4182406..4183791 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  SA976_RS20520 (SA976_20520) hofC 4183781..4184983 (+) 1203 WP_000157234.1 protein transport protein HofC -
  SA976_RS20525 (SA976_20525) guaC 4185018..4186061 (-) 1044 WP_001217338.1 GMP reductase -
  SA976_RS20530 (SA976_20530) - 4186217..4186261 (-) 45 WP_120795372.1 protein YacM -
  SA976_RS20535 (SA976_20535) coaE 4186286..4186906 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  SA976_RS20540 (SA976_20540) zapD 4186906..4187649 (+) 744 WP_001194734.1 cell division protein ZapD -
  SA976_RS20545 (SA976_20545) yacG 4187659..4187856 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  SA976_RS20550 (SA976_20550) mutT 4187956..4188345 (-) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50618.29 Da        Isoelectric Point: 6.5786

>NTDB_id=824059 SA976_RS20515 WP_001025155.1 4182406..4183791(+) (pilB) [Escherichia coli strain GCEC19]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=824059 SA976_RS20515 WP_001025155.1 4182406..4183791(+) (pilB) [Escherichia coli strain GCEC19]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTGTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.65

100

0.447
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36