Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SA976_RS20510 Genome accession   NZ_CP137765
Coordinates   4181956..4182396 (+) Length   146 a.a.
NCBI ID   WP_000360894.1    Uniprot ID   -
Organism   Escherichia coli strain GCEC19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4176956..4187396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA976_RS20490 (SA976_20490) aroP 4177954..4179327 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  SA976_RS20495 (SA976_20495) ampE 4179370..4180224 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  SA976_RS20500 (SA976_20500) ampD 4180221..4180772 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SA976_RS20505 (SA976_20505) nadC 4180860..4181753 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SA976_RS20510 (SA976_20510) pilA 4181956..4182396 (+) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  SA976_RS20515 (SA976_20515) pilB 4182406..4183791 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  SA976_RS20520 (SA976_20520) hofC 4183781..4184983 (+) 1203 WP_000157234.1 protein transport protein HofC -
  SA976_RS20525 (SA976_20525) guaC 4185018..4186061 (-) 1044 WP_001217338.1 GMP reductase -
  SA976_RS20530 (SA976_20530) - 4186217..4186261 (-) 45 WP_120795372.1 protein YacM -
  SA976_RS20535 (SA976_20535) coaE 4186286..4186906 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15611.72 Da        Isoelectric Point: 4.5538

>NTDB_id=824058 SA976_RS20510 WP_000360894.1 4181956..4182396(+) (pilA) [Escherichia coli strain GCEC19]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=824058 SA976_RS20510 WP_000360894.1 4181956..4182396(+) (pilA) [Escherichia coli strain GCEC19]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377