Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QC632_RS22110 Genome accession   NZ_CP123897
Coordinates   5006165..5007880 (-) Length   571 a.a.
NCBI ID   WP_281021547.1    Uniprot ID   -
Organism   Methylomonas sp. UP202     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5001165..5012880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QC632_RS22080 (QC632_22080) - 5001508..5002464 (+) 957 WP_281021541.1 Nudix family hydrolase -
  QC632_RS22085 (QC632_22085) yacG 5002449..5002652 (-) 204 WP_281021542.1 DNA gyrase inhibitor YacG -
  QC632_RS22090 (QC632_22090) zapD 5002652..5003422 (-) 771 WP_281021543.1 cell division protein ZapD -
  QC632_RS22095 (QC632_22095) coaE 5003419..5004030 (-) 612 WP_281021544.1 dephospho-CoA kinase -
  QC632_RS22100 (QC632_22100) pilD 5004030..5004896 (-) 867 WP_281021545.1 A24 family peptidase Machinery gene
  QC632_RS22105 (QC632_22105) pilC 5004945..5006162 (-) 1218 WP_281021546.1 type II secretion system F family protein Machinery gene
  QC632_RS22110 (QC632_22110) pilB 5006165..5007880 (-) 1716 WP_281021547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QC632_RS22115 (QC632_22115) thiC 5008029..5009897 (-) 1869 WP_281021548.1 phosphomethylpyrimidine synthase ThiC -
  QC632_RS22120 (QC632_22120) recD 5010175..5011809 (+) 1635 WP_281021549.1 exodeoxyribonuclease V subunit alpha -
  QC632_RS22125 (QC632_22125) - 5011806..5012225 (+) 420 WP_281021550.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63102.72 Da        Isoelectric Point: 6.2552

>NTDB_id=823009 QC632_RS22110 WP_281021547.1 5006165..5007880(-) (pilB) [Methylomonas sp. UP202]
MATAPSNIHFSGLAKCLIQKGLLTEETAAAYLQEAQKRKQAFVSYLVEHKVLDSRTVASIASAEFGVPFFDLDAIDVASL
PIKMVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLMKMIDTALDAADTSMKDLLDD
DLDNIDISAGDEAKPAEEVGGSDIDDAPIVRFVNKILLDSIKRGVSDIHMEPFEKKFRIRFRADGILSEVASPPVGIANR
IISRIKVMSKMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVMRILDPTSAQIGIEKLGFEPVQQKNFLDAI
HRPYGMVLVTGPTGSGKTVSLYTGLNILNSVERNISTAEDPVEITVEGINQVNVNAKAGLTFAEALRAFLRQDPDIIMVG
EIRDLETASIAVKAAQTGHLVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVNLIMAQRLARRLCEHCKKEADFPENIWLS
AGFSKEEIGTFKIYEPVGCEHCNNGYKGRVGIYQVMPISEAMRALILRGGNAMDMAALSKTEGINDLRASGLLKIRQGIT
SLEEIDRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=823009 QC632_RS22110 WP_281021547.1 5006165..5007880(-) (pilB) [Methylomonas sp. UP202]
ATGGCGACAGCTCCTTCCAATATTCATTTCAGCGGCTTGGCCAAGTGCCTGATCCAAAAAGGCCTGTTGACTGAAGAGAC
TGCCGCCGCCTATCTGCAAGAAGCCCAAAAACGCAAGCAAGCCTTCGTCAGTTATCTGGTCGAGCATAAAGTGCTCGACA
GTCGGACCGTGGCCTCCATCGCTTCCGCCGAGTTCGGCGTGCCGTTTTTCGATTTGGATGCGATAGATGTGGCGTCGCTG
CCTATCAAAATGGTCAGCGAAAAGCTTATCCGCCAACATCACGCTTTGCCACTGTTCAAACGCGGTAACCGCTTGTTCGT
CGCCACCGCCGATCCGACCAATTTCCAGGCACTCGACGAAATCAAGTTCCATACTCGGCACAATACCGAGAACATTTTGG
TCGAAGAAGACAAATTGATGAAGATGATCGATACCGCACTGGATGCGGCCGATACATCAATGAAGGATTTGCTCGACGAC
GACCTGGACAACATCGATATCTCCGCCGGCGACGAAGCCAAGCCGGCCGAGGAAGTCGGCGGTTCGGACATCGACGATGC
GCCCATCGTCCGTTTCGTCAACAAAATCCTGCTCGATTCGATCAAGCGCGGCGTCTCGGATATACACATGGAGCCGTTCG
AGAAAAAGTTTCGGATCCGGTTCCGGGCCGACGGCATTTTGTCCGAAGTCGCCAGTCCGCCGGTCGGCATTGCCAATCGG
ATCATATCTCGGATCAAGGTCATGTCGAAAATGGACATCGCCGAGCGGCGGGTGCCCCAGGACGGCCGGATCAAAATGCA
AATTTCCAAGAATCGGGCCATCGATTTCCGGGTGAATACCTGTCCGACGCTGTTCGGCGAAAAAGTGGTGATGCGGATAC
TCGATCCCACCAGCGCCCAGATCGGTATCGAAAAATTGGGTTTCGAGCCTGTTCAACAAAAAAACTTTTTGGACGCCATT
CACCGGCCCTACGGGATGGTGTTGGTGACCGGGCCAACCGGTAGCGGTAAGACGGTGTCGCTGTACACCGGCTTGAACAT
TCTGAACAGCGTCGAGCGGAACATTTCGACCGCCGAAGATCCGGTCGAGATCACGGTCGAAGGTATCAATCAGGTCAACG
TCAATGCCAAAGCCGGTTTGACCTTCGCCGAAGCCTTGCGGGCTTTTTTGCGGCAAGACCCGGATATCATCATGGTCGGC
GAGATCCGCGACCTGGAAACCGCCAGCATCGCGGTCAAGGCAGCGCAAACCGGTCACTTGGTATTGTCGACCCTGCACAC
CAACGACGCGCCGCAAACCATCAATCGCTTGGTGCAGATGGGGATAGAGCCGTTCAATATCGTGTCGGCGGTGAATCTGA
TCATGGCCCAGCGCTTGGCGCGGCGGCTTTGCGAGCATTGCAAGAAGGAGGCCGACTTTCCGGAAAATATCTGGTTGTCG
GCCGGCTTTAGTAAGGAAGAAATCGGCACCTTCAAAATTTACGAACCGGTCGGTTGCGAGCATTGCAACAATGGCTACAA
GGGGCGGGTGGGTATCTATCAGGTGATGCCGATCAGCGAAGCGATGCGCGCGTTGATTTTGCGTGGCGGCAATGCCATGG
ACATGGCCGCGCTGTCCAAAACCGAAGGCATCAACGACCTTAGGGCTTCCGGCTTATTAAAAATACGGCAGGGTATTACG
TCGCTCGAAGAAATCGACCGCGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

52.356

100

0.525
  pilB Acinetobacter baylyi ADP1

52.66

98.774

0.52
  pilF Neisseria gonorrhoeae MS11

48.208

97.723

0.471
  pilB Vibrio cholerae strain A1552

46.549

98.949

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

45.907

98.424

0.452
  pilB Vibrio campbellii strain DS40M4

45.133

98.949

0.447
  pilF Thermus thermophilus HB27

39.427

97.723

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.503

98.249

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.909

96.322

0.375