Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   R6K63_RS27365 Genome accession   NZ_CP137556
Coordinates   5774017..5774556 (-) Length   179 a.a.
NCBI ID   WP_031285592.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CDC1270     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5769017..5779556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6K63_RS27330 fliP 5769628..5770383 (+) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -
  R6K63_RS27335 fliQ 5770428..5770697 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  R6K63_RS27340 fliR 5770697..5771479 (+) 783 Protein_5418 flagellar biosynthetic protein FliR -
  R6K63_RS27345 - 5771482..5771741 (+) 260 Protein_5419 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  R6K63_RS27350 - 5772770..5773012 (+) 243 WP_023082027.1 hypothetical protein -
  R6K63_RS27360 - 5773255..5773953 (-) 699 WP_016254081.1 hypothetical protein -
  R6K63_RS27365 pilE 5774017..5774556 (-) 540 WP_031285592.1 pilin Machinery gene
  R6K63_RS27370 pilB 5774785..5776488 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R6K63_RS27375 pilC 5776492..5777709 (+) 1218 WP_023435364.1 type II secretion system F family protein Machinery gene
  R6K63_RS27380 pilD 5777712..5778584 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R6K63_RS27385 coaE 5778581..5779192 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  R6K63_RS27390 yacG 5779189..5779389 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18084.43 Da        Isoelectric Point: 4.7718

>NTDB_id=822381 R6K63_RS27365 WP_031285592.1 5774017..5774556(-) (pilE) [Pseudomonas aeruginosa strain CDC1270]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGDAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASESNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=822381 R6K63_RS27365 WP_031285592.1 5774017..5774556(-) (pilE) [Pseudomonas aeruginosa strain CDC1270]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTGACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.243

100

0.447
  pilA2 Legionella pneumophila str. Paris

41.477

98.324

0.408
  pilA2 Legionella pneumophila strain ERS1305867

40.909

98.324

0.402
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363