Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QAO71_RS13065 Genome accession   NZ_CP123445
Coordinates   2771376..2773082 (+) Length   568 a.a.
NCBI ID   WP_280164637.1    Uniprot ID   -
Organism   Halopseudomonas sp. SMJS2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2766376..2778082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO71_RS13045 (QAO71_13045) - 2767593..2767985 (+) 393 WP_143063021.1 type IV pilin protein -
  QAO71_RS13050 (QAO71_13050) - 2768198..2769247 (-) 1050 WP_280164634.1 acyltransferase -
  QAO71_RS13055 (QAO71_13055) - 2769244..2770443 (-) 1200 WP_280164635.1 serine hydrolase domain-containing protein -
  QAO71_RS13060 (QAO71_13060) - 2770521..2770982 (-) 462 WP_280164636.1 pilin -
  QAO71_RS13065 (QAO71_13065) pilB 2771376..2773082 (+) 1707 WP_280164637.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QAO71_RS13070 (QAO71_13070) pilC 2773086..2774354 (+) 1269 WP_280164638.1 type II secretion system F family protein Machinery gene
  QAO71_RS13075 (QAO71_13075) pilD 2774358..2775230 (+) 873 WP_280164639.1 A24 family peptidase Machinery gene
  QAO71_RS13080 (QAO71_13080) coaE 2775529..2776152 (+) 624 WP_280164640.1 dephospho-CoA kinase -
  QAO71_RS13085 (QAO71_13085) yacG 2776149..2776337 (+) 189 WP_036988440.1 DNA gyrase inhibitor YacG -
  QAO71_RS13090 (QAO71_13090) - 2776369..2777415 (+) 1047 WP_280164641.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62670.60 Da        Isoelectric Point: 5.1631

>NTDB_id=821141 QAO71_RS13065 WP_280164637.1 2771376..2773082(+) (pilB) [Halopseudomonas sp. SMJS2]
MDTPALSGLARRIVQEELLDAQAAGKASQQASQDKIPLITYLVQNKLAKARDLAMVCAEEFGYPYLDLTALDRDSQPEVS
LVSEKLVRQHCVMPLYKRGSRLYLALSDPTNQQALSDIQFNTGLMTDAVIVEDDKLRTAIDKYLESATGGLGDMDDGDLD
SLEVESAADDDKRKDAAGDTEDAPVVRFVNKMLLDAVKKGSSDLHFEPYEKTYRVRFRTDGVLHEVSKPPVQLGVKIAAR
LKVMSSMDLAERRKPQDGRIKLKISKNKAIDFRVNTLPTLWGEKVVLRILDADSAKMGIDALGYEDDQKQMYLDALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTMERNISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVASFNIATSVNLIIAQRLARRLCSHCKKPMDIPRETLREEGYS
EEKIASGLTIYGPVGCENCQDGYKGRVGIYEVVRITPAIQRIIMEDGNSMQISDVAQNEGFRSLRQSALMKAEQGVTSLA
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=821141 QAO71_RS13065 WP_280164637.1 2771376..2773082(+) (pilB) [Halopseudomonas sp. SMJS2]
ATGGATACCCCCGCTCTCTCCGGCCTGGCCCGGCGGATTGTTCAGGAAGAACTGCTGGATGCGCAGGCGGCTGGCAAGGC
CAGTCAACAGGCCAGCCAGGACAAGATTCCCCTCATTACTTATCTGGTGCAGAACAAGCTGGCCAAGGCGCGTGACCTGG
CCATGGTATGTGCCGAGGAGTTCGGCTACCCCTATCTGGACCTGACTGCACTGGACCGCGACAGCCAGCCGGAGGTCAGC
CTGGTTAGCGAGAAACTGGTCCGCCAACATTGCGTGATGCCCTTGTATAAGCGCGGTTCGCGACTGTATTTGGCGCTGTC
CGATCCGACCAATCAGCAGGCACTGAGCGATATCCAATTTAATACTGGTTTGATGACCGATGCGGTGATTGTCGAGGATG
ACAAGCTGCGCACGGCCATTGACAAGTACTTGGAGAGCGCGACCGGTGGCCTGGGCGATATGGACGATGGCGACCTTGAT
AGCCTTGAAGTTGAAAGCGCTGCCGATGACGATAAGCGCAAGGATGCGGCTGGCGACACGGAAGACGCACCCGTGGTGCG
CTTCGTCAACAAGATGCTGTTGGATGCGGTGAAGAAAGGCTCCTCGGACTTGCACTTCGAGCCCTACGAGAAGACCTACC
GGGTGCGTTTTCGTACCGACGGCGTGTTGCATGAGGTATCCAAACCGCCGGTGCAATTGGGGGTGAAGATTGCTGCGCGT
TTGAAGGTTATGTCTTCGATGGACCTGGCCGAACGTCGCAAGCCACAGGATGGTCGCATCAAGCTGAAGATCTCCAAGAA
CAAGGCCATCGACTTCCGGGTCAACACCCTGCCGACGCTGTGGGGCGAGAAAGTGGTGCTGCGTATTCTCGATGCGGACA
GTGCCAAGATGGGTATTGATGCGCTGGGTTATGAGGATGACCAGAAGCAGATGTATCTGGATGCGCTGAGTAAGCCCCAG
GGCATGATTTTGGTCACCGGCCCAACCGGCTCCGGTAAGACGGTGTCGCTGTATACCGGTCTGAACATTCTCAACACCAT
GGAGCGCAATATCTCCACCGCAGAAGACCCGGTGGAGATCAACCTAGAGGGGATCAACCAGGTCAACGTGAATCCCAAGC
AGGGACTGGATTTTGCCCAGGCTCTGCGTGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGTGAGATTCGTGAC
CTGGAGACGGCCGAGATTGCCATCAAGGCGGCGCAGACCGGTCACATGGTCATGTCCACTCTGCACACCAACAGCGCGGC
AGAAACCCTTACCCGCCTGCGCAACATGGGTGTGGCCTCATTCAATATCGCCACCTCGGTAAACCTGATCATTGCTCAGC
GCCTGGCGCGGCGGCTGTGTTCACACTGCAAGAAGCCGATGGATATTCCCCGCGAGACGCTGCGCGAGGAAGGCTACTCT
GAAGAAAAAATTGCTAGCGGGTTAACGATTTACGGGCCGGTGGGTTGCGAAAATTGTCAGGACGGTTATAAAGGGCGTGT
CGGGATTTATGAAGTGGTCAGAATCACGCCGGCCATCCAGCGCATCATCATGGAAGACGGTAACTCCATGCAGATTTCCG
ACGTAGCCCAGAATGAAGGATTCAGAAGCTTGCGCCAATCCGCGCTGATGAAGGCGGAACAGGGTGTCACCAGCCTGGCG
GAAGTGAACCGAGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.227

100

0.593
  pilB Acinetobacter baumannii D1279779

58.947

100

0.592
  pilB Legionella pneumophila strain ERS1305867

56.514

100

0.565
  pilF Neisseria gonorrhoeae MS11

49.823

99.648

0.496
  pilB Vibrio cholerae strain A1552

49.556

99.12

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

53.557

89.085

0.477
  pilB Vibrio campbellii strain DS40M4

53.388

85.739

0.458
  pilF Thermus thermophilus HB27

38.721

99.12

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

93.662

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

40.954

88.556

0.363