Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QAO71_RS13075 Genome accession   NZ_CP123445
Coordinates   2774358..2775230 (+) Length   290 a.a.
NCBI ID   WP_280164639.1    Uniprot ID   -
Organism   Halopseudomonas sp. SMJS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2769358..2780230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO71_RS13060 (QAO71_13060) - 2770521..2770982 (-) 462 WP_280164636.1 pilin -
  QAO71_RS13065 (QAO71_13065) pilB 2771376..2773082 (+) 1707 WP_280164637.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QAO71_RS13070 (QAO71_13070) pilC 2773086..2774354 (+) 1269 WP_280164638.1 type II secretion system F family protein Machinery gene
  QAO71_RS13075 (QAO71_13075) pilD 2774358..2775230 (+) 873 WP_280164639.1 A24 family peptidase Machinery gene
  QAO71_RS13080 (QAO71_13080) coaE 2775529..2776152 (+) 624 WP_280164640.1 dephospho-CoA kinase -
  QAO71_RS13085 (QAO71_13085) yacG 2776149..2776337 (+) 189 WP_036988440.1 DNA gyrase inhibitor YacG -
  QAO71_RS13090 (QAO71_13090) - 2776369..2777415 (+) 1047 WP_280164641.1 Nudix family hydrolase -
  QAO71_RS13095 (QAO71_13095) ispH 2777455..2778402 (-) 948 WP_280164642.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  QAO71_RS13100 (QAO71_13100) fkpB 2778437..2778874 (-) 438 WP_036990253.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  QAO71_RS13105 (QAO71_13105) lspA 2778976..2779470 (-) 495 WP_280164643.1 signal peptidase II -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32206.22 Da        Isoelectric Point: 7.3699

>NTDB_id=821143 QAO71_RS13075 WP_280164639.1 2774358..2775230(+) (pilD) [Halopseudomonas sp. SMJS2]
MTLLDYLASQLLAFVVVAGLLGLVVGSFLNVVIHRLPKMMERDWLQQAREMLHPEQPQTEQPVYNLVLPHSHCPHCQTEI
KPWQNLPLISYALLRGRCGSCRERISPRYPLVELLTAVLSIAVAWQFGFGWPAAGMLLLTWTLIALSLIDADTQLLPDAI
VLPMLWLGLIANSFGLFTDMHSALRGAVFGYLSLWSVYWLFKLITGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLV
GAILGIIILKTRGDSNSTPLPFGPYLAIAGWIAIIWGDWITGTYLRFAGF

Nucleotide


Download         Length: 873 bp        

>NTDB_id=821143 QAO71_RS13075 WP_280164639.1 2774358..2775230(+) (pilD) [Halopseudomonas sp. SMJS2]
ATGACCTTACTTGATTATTTGGCCAGCCAGCTGCTGGCCTTTGTTGTAGTCGCTGGGCTGCTGGGATTGGTAGTTGGCAG
CTTTCTCAATGTGGTGATTCATCGCTTGCCGAAGATGATGGAGCGCGACTGGCTGCAACAGGCCCGGGAGATGTTGCATC
CGGAGCAGCCACAGACCGAGCAGCCGGTGTACAACCTGGTGCTGCCTCATTCCCACTGTCCGCATTGCCAGACCGAGATC
AAACCCTGGCAGAATCTGCCGTTGATCAGCTATGCGCTGTTGCGTGGCCGCTGCGGCAGTTGCCGGGAGCGTATCAGTCC
GCGCTATCCACTGGTCGAGCTGCTCACCGCCGTGCTGAGTATCGCGGTAGCCTGGCAATTCGGCTTCGGCTGGCCGGCCG
CCGGGATGCTACTGCTGACCTGGACGCTGATTGCCCTCAGCCTGATCGATGCCGATACCCAATTGCTGCCGGATGCCATT
GTGCTGCCGATGTTGTGGCTGGGGTTGATTGCCAATAGCTTCGGGTTGTTCACCGATATGCATTCGGCGCTCCGGGGCGC
AGTGTTCGGCTATCTTAGCCTGTGGTCGGTGTATTGGCTGTTCAAACTGATCACCGGCAAGGAAGGCATGGGCTATGGCG
ACTTCAAGCTACTGGCGATGCTCGGAGCCTGGGGTGGTTGGCAGGTGTTGCCGTTGACCATCTTGCTGTCCTCGCTGGTG
GGTGCGATTCTCGGGATCATTATTCTGAAGACTCGCGGCGACTCGAATTCCACCCCCCTGCCCTTCGGACCTTATTTGGC
CATTGCCGGGTGGATTGCGATTATCTGGGGGGATTGGATTACTGGAACGTATTTGCGGTTTGCGGGGTTTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-246)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

53.846

98.621

0.531
  pilD Acinetobacter nosocomialis M2

53.497

98.621

0.528
  pilD Vibrio cholerae strain A1552

52.747

94.138

0.497
  pilD Vibrio campbellii strain DS40M4

50.549

94.138

0.476
  pilD Neisseria gonorrhoeae MS11

51.515

91.034

0.469