Detailed information NTG0817684

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUL033_RS09410
• Genome accession: NZ_CP122980
• Coordinates: 1883756..1884412 (-)
• Length: 218 a.a.
• NCBI ID: WP_014735120.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain UL033
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1878756..1889412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUL033_RS09385 (LLUL033_09395)	atpF	1878827..1879333 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLUL033_RS09390 (LLUL033_09400)	atpB	1879348..1880061 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLUL033_RS09395 (LLUL033_09405)	-	1880108..1880323 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLUL033_RS09400 (LLUL033_09410)	-	1880506..1881282 (-)	777	WP_011835694.1	alpha/beta hydrolase family protein	-
LLUL033_RS09405 (LLUL033_09415)	-	1881566..1883775 (-)	2210	Protein_1833	DNA internalization-related competence protein ComEC/Rec2	-
LLUL033_RS09410 (LLUL033_09420)	comEA	1883756..1884412 (-)	657	WP_014735120.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLUL033_RS09415 (LLUL033_09425)	-	1884470..1885831 (-)	1362	WP_011835696.1	ABC transporter permease	-
LLUL033_RS09420 (LLUL033_09430)	-	1885828..1886760 (-)	933	WP_011835697.1	ABC transporter ATP-binding protein	-
LLUL033_RS09425 (LLUL033_09435)	-	1886866..1887264 (-)	399	WP_042747837.1	hypothetical protein	-
LLUL033_RS09430 (LLUL033_09440)	-	1887381..1887941 (-)	561	WP_011835699.1	GNAT family N-acetyltransferase	-
LLUL033_RS09435 (LLUL033_09445)	-	1888115..1889293 (-)	1179	WP_014735121.1	SLC13 family permease	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 23674.86 Da
• Isoelectric Point: 5.1730

>NTDB_id=817684 LLUL033_RS09410 WP_014735120.1 1883756..1884412(-) (comEA) [Lactococcus cremoris strain UL033]
MEKILDKLKEYWKIMLLVVCALIAGGILYIFTNSPKPAETLSIENLSSSSTKSSVSKFNSSSSEKNKNEIMVDLKGAVAK
PNVYQISSDERLVDLIRQAGGFTDQADPKSINLSAKLKDEEVIYVPKVGESSSSESTDSPTGSSVSNQVSTTSGPKVNIN
KADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 657 bp

>NTDB_id=817684 LLUL033_RS09410 WP_014735120.1 1883756..1884412(-) (comEA) [Lactococcus cremoris strain UL033]
ATGGAAAAAATTTTAGATAAATTAAAAGAATACTGGAAAATAATGCTTTTAGTTGTTTGTGCACTTATTGCTGGTGGAAT
TCTTTATATTTTCACCAATTCACCAAAGCCAGCTGAAACTCTGTCAATAGAAAATTTGAGCTCTAGTTCTACCAAAAGCT
CTGTCAGTAAATTTAATAGTAGCAGTAGTGAAAAAAATAAAAATGAAATTATGGTTGATTTAAAAGGAGCGGTTGCAAAA
CCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGA
CCCAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAAGTTGGGGAAAGTTCAAGTT
CAGAAAGTACTGACAGTCCTACTGGTAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAGTAAATATCAAT
AAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAA
AAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAACTGAAAGATG
AGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (71-126)

• Helix-hairpin-helix motif (HHH_3) (154-215)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	98.165	100	0.982
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	41.41	100	0.431
comEA/celA/cilE	Streptococcus mitis SK321	41.778	100	0.431
comEA/celA/cilE	Streptococcus mitis NCTC 12261	40.529	100	0.422
comEA/celA/cilE	Streptococcus pneumoniae R6	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae Rx1	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae D39	40.991	100	0.417
comEA	Latilactobacillus sakei subsp. sakei 23K	37.021	100	0.399
comEA	Staphylococcus aureus MW2	35.931	100	0.381
comEA	Staphylococcus aureus N315	36.866	99.541	0.367