Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PA12_RS26745 Genome accession   NZ_CP136598
Coordinates   5716651..5717115 (-) Length   154 a.a.
NCBI ID   WP_211851992.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PA12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5711651..5722115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA12_RS26730 (PA12_gene5217) nadC 5714151..5714999 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PA12_RS26740 (PA12_gene5218) - 5715180..5716493 (-) 1314 WP_223697243.1 O-antigen ligase family protein -
  PA12_RS26745 (PA12_gene5219) pilA/pilAI 5716651..5717115 (-) 465 WP_211851992.1 pilin Machinery gene
  PA12_RS26750 (PA12_gene5220) pilB 5717346..5719046 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PA12_RS26755 (PA12_gene5221) pilC 5719050..5720267 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  PA12_RS26760 (PA12_gene5222) pilD 5720268..5721140 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PA12_RS26765 (PA12_gene5223) coaE 5721137..5721748 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PA12_RS26770 (PA12_gene5224) yacG 5721745..5721945 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16160.52 Da        Isoelectric Point: 8.9986

>NTDB_id=815221 PA12_RS26745 WP_211851992.1 5716651..5717115(-) (pilA/pilAI) [Pseudomonas aeruginosa strain PA12]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWICGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=815221 PA12_RS26745 WP_211851992.1 5716651..5717115(-) (pilA/pilAI) [Pseudomonas aeruginosa strain PA12]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGATCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.208

100

0.422
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

36.471

100

0.403
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.411

98.052

0.377
  pilA Vibrio cholerae C6706

38.411

98.052

0.377
  pilA Vibrio cholerae strain A1552

38.411

98.052

0.377