Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS04_RS01085 Genome accession   NZ_CP121854
Coordinates   216153..217859 (-) Length   568 a.a.
NCBI ID   WP_279465677.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 13997     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 211153..222859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS04_RS01055 mutT 211664..212068 (+) 405 WP_107684321.1 8-oxo-dGTP diphosphatase MutT -
  QBS04_RS01060 yacG 212146..212340 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS04_RS01065 zapD 212350..213072 (-) 723 WP_005336141.1 cell division protein ZapD -
  QBS04_RS01070 coaE 213110..213724 (-) 615 WP_107684319.1 dephospho-CoA kinase -
  QBS04_RS01075 pilD 213738..214610 (-) 873 WP_107684318.1 A24 family peptidase Machinery gene
  QBS04_RS01080 pilC 214672..215913 (-) 1242 WP_005355091.1 type II secretion system F family protein Machinery gene
  QBS04_RS01085 pilB 216153..217859 (-) 1707 WP_279465677.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS04_RS01090 tapA 217861..218343 (-) 483 WP_202004473.1 type IVa pilus major pilin TapA -
  QBS04_RS01095 nadC 218672..219547 (-) 876 WP_219594802.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS04_RS01100 - 219551..220021 (-) 471 WP_219594801.1 retropepsin-like aspartic protease -
  QBS04_RS01105 ampD 220152..220727 (+) 576 WP_279465678.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS04_RS01110 pdhR 221006..221770 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62604.91 Da        Isoelectric Point: 5.2237

>NTDB_id=814788 QBS04_RS01085 WP_279465677.1 216153..217859(-) (pilB) [Aeromonas veronii strain ANYA 13997]
MNSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKEQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814788 QBS04_RS01085 WP_279465677.1 216153..217859(-) (pilB) [Aeromonas veronii strain ANYA 13997]
ATGAACTCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATTAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATCGAAAACGAGATCCTCGATAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCACTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTCTCCGACGAAAACAGTCGGCTGGACGAGAGCGTCAATACTGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAAATCCTGATGGACGCCATCAAGCGTGGTGCCTCCGACCTACACTTCGAGCCCTATGAGTTCAAGTATC
GGGTCCGCTTTCGCATCGATGGCATTCTGCATGAGATCGCCACACCGCCGGTTAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTGATGGCGCGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGAGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCTTGCGATCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCCATCAAGGCTGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTACCGGCTTTCAACATAGCCTCCTCGGTAACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTATGTGACCACTGCAAAGCGCCGGAAGTGGTACCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGTTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATCGCCG
CGATCGCCCAGAAGGAAGGGATGCGCACCCTGCGCACCTCGGGGCTGGAGAAGGCCCGCCTCGGTGTCACCAGTCTGGCC
GAGGTCAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.614

100

0.558
  pilB Acinetobacter baylyi ADP1

55.95

99.12

0.555
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.72

92.958

0.379