Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS11_RS01000 Genome accession   NZ_CP121851
Coordinates   197958..199664 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 14022     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 192958..204664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS11_RS00970 mutT 193468..193872 (+) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -
  QBS11_RS00975 yacG 193950..194144 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS11_RS00980 zapD 194154..194876 (-) 723 WP_005336141.1 cell division protein ZapD -
  QBS11_RS00985 coaE 194914..195528 (-) 615 WP_058058638.1 dephospho-CoA kinase -
  QBS11_RS00990 pilD 195542..196414 (-) 873 WP_058058639.1 A24 family peptidase Machinery gene
  QBS11_RS00995 pilC 196476..197717 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  QBS11_RS01000 pilB 197958..199664 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS11_RS01005 tapA 199668..200108 (-) 441 WP_320606780.1 type IVa pilus major pilin TapA -
  QBS11_RS01010 nadC 200424..201287 (-) 864 WP_058057549.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS11_RS01015 - 201291..201761 (-) 471 WP_279491554.1 retropepsin-like aspartic protease -
  QBS11_RS01020 ampD 201892..202467 (+) 576 WP_058057550.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS11_RS01025 pdhR 202746..203510 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=814761 QBS11_RS01000 WP_005336134.1 197958..199664(-) (pilB) [Aeromonas veronii strain ANYA 14022]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814761 QBS11_RS01000 WP_005336134.1 197958..199664(-) (pilB) [Aeromonas veronii strain ANYA 14022]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAAAACGAGATCCTCGACAGCAAGGCGCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAAGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTCGGCTTCAGCTTTGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCTGATCTCGAGGTCTCTGACGAGAACAGCCGCCTCGACGAAAGCGTTAATACCGCCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGTGGTGCCTCCGACCTGCACTTCGAGCCATATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGACGGTATTCTGCATGAAATTGCTACCCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTGATGGCGCGGCTCGATATCGCCGAGCGGCGGCTGCCGCAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAACATCTCCACCGCCGAAGATCCGGTAGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CAGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTTTTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCGGCACAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
AGAGACCCTGACCCGAATGATGAACATGGGGGTGCCCGCGTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTGTGCGACCACTGCAAGGCACCGGAAGTTGTGCCGGAAGCAGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAACTGGCGGCCGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATCGCAG
CTATCGCCCAGAAGGAAGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377