Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS12_RS04240 Genome accession   NZ_CP121841
Coordinates   888266..889972 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18196     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 883266..894972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS12_RS04210 mutT 883775..884179 (+) 405 WP_279464201.1 8-oxo-dGTP diphosphatase MutT -
  QBS12_RS04215 yacG 884258..884452 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS12_RS04220 zapD 884462..885184 (-) 723 WP_021231281.1 cell division protein ZapD -
  QBS12_RS04225 coaE 885222..885836 (-) 615 WP_279464202.1 dephospho-CoA kinase -
  QBS12_RS04230 pilD 885850..886722 (-) 873 WP_279464203.1 A24 family peptidase Machinery gene
  QBS12_RS04235 pilC 886784..888025 (-) 1242 WP_111899845.1 type II secretion system F family protein Machinery gene
  QBS12_RS04240 pilB 888266..889972 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS12_RS04245 - 889982..890428 (-) 447 WP_236271771.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS12_RS04250 nadC 890744..891607 (-) 864 WP_279464204.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS12_RS04255 - 891611..892081 (-) 471 WP_040068932.1 retropepsin-like aspartic protease -
  QBS12_RS04260 ampD 892212..892787 (+) 576 WP_069554218.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS12_RS04265 pdhR 893066..893830 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=814578 QBS12_RS04240 WP_005336134.1 888266..889972(-) (pilB) [Aeromonas veronii strain ANYA 18196]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814578 QBS12_RS04240 WP_005336134.1 888266..889972(-) (pilB) [Aeromonas veronii strain ANYA 18196]
ATGACTTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAGCCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCTTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAACTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAAGTCTCCGACGAAAACAGCCGGCTGGACGAAAGTGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCTTACGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTCTGCACGAGATCGCCACACCGCCGGTCAATCTCTCCAACCGCTTCTCAGCACGC
CTAAAAGTCATGGCGCGACTCGATATCGCCGAGCGGCGGCTACCGCAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTTAATACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGTTTTGATGACCGGCAGAAAGCGCAGTACCTGCGCGCCCTCTCGAAACCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTAAATATTCTCAACACCAC
CGAGGTAAATATCTCCACCGCCGAAGACCCGGTAGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCTTGCGCTCCTTCTTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCGGCGCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGAGTACCGGCCTTCAACATCGCTTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTACCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGTACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGTGTCACCAGTCTGGCC
GAGGTCAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377