Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS27_RS00795 Genome accession   NZ_CP121840
Coordinates   159937..161643 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18197     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 154937..166643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS27_RS00765 mutT 155456..155860 (+) 405 WP_167573346.1 8-oxo-dGTP diphosphatase MutT -
  QBS27_RS00770 yacG 155939..156133 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS27_RS00775 zapD 156143..156865 (-) 723 WP_005336141.1 cell division protein ZapD -
  QBS27_RS00780 coaE 156903..157517 (-) 615 WP_100652537.1 dephospho-CoA kinase -
  QBS27_RS00785 pilD 157531..158403 (-) 873 WP_088868329.1 A24 family peptidase Machinery gene
  QBS27_RS00790 pilC 158465..159706 (-) 1242 WP_139433411.1 type II secretion system F family protein Machinery gene
  QBS27_RS00795 pilB 159937..161643 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS27_RS00800 - 161647..162102 (-) 456 WP_201943837.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS27_RS00805 nadC 162433..163293 (-) 861 WP_279490123.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS27_RS00810 - 163297..163767 (-) 471 WP_005336129.1 retropepsin-like aspartic protease -
  QBS27_RS00815 ampD 163898..164473 (+) 576 WP_279490124.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS27_RS00820 pdhR 164752..165516 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=814543 QBS27_RS00795 WP_005336134.1 159937..161643(-) (pilB) [Aeromonas veronii strain ANYA 18197]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814543 QBS27_RS00795 WP_005336134.1 159937..161643(-) (pilB) [Aeromonas veronii strain ANYA 18197]
ATGACCTCTAGTCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGACTTTTGCGAACTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTACCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGATGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGTCGGCTGGACGAAAGCGTCAATACCGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCAATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTACGAGTTCAAGTATC
GGGTGCGTTTTCGCATCGATGGCATTCTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTGATGGCGCGACTCGATATTGCCGAGCGGCGGTTGCCGCAGGATGGCCGCATCAAGTTGAAGTTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTTAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAACCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTGAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGTGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCTATCAAGGCGGCGCAGACCGGTCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
AGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAACTGGCTGCCGGACTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCACGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377