Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS07_RS02240 Genome accession   NZ_CP121837
Coordinates   471469..473175 (+) Length   568 a.a.
NCBI ID   WP_279488476.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18263     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466469..478175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS07_RS02215 pdhR 467619..468383 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QBS07_RS02220 ampD 468662..469237 (-) 576 WP_279488474.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS07_RS02225 - 469368..469838 (+) 471 WP_005336129.1 retropepsin-like aspartic protease -
  QBS07_RS02230 nadC 469842..470705 (+) 864 WP_279488475.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS07_RS02235 tapA 471033..471459 (+) 427 Protein_415 type IVa pilus major pilin TapA -
  QBS07_RS02240 pilB 471469..473175 (+) 1707 WP_279488476.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS07_RS02245 pilC 473416..474657 (+) 1242 WP_111899845.1 type II secretion system F family protein Machinery gene
  QBS07_RS02250 pilD 474719..475591 (+) 873 WP_279488477.1 A24 family peptidase Machinery gene
  QBS07_RS02255 coaE 475605..476219 (+) 615 WP_279488478.1 dephospho-CoA kinase -
  QBS07_RS02260 zapD 476257..476979 (+) 723 WP_279488479.1 cell division protein ZapD -
  QBS07_RS02265 yacG 476989..477183 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS07_RS02270 mutT 477261..477665 (-) 405 WP_279488480.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62535.85 Da        Isoelectric Point: 5.3170

>NTDB_id=814515 QBS07_RS02240 WP_279488476.1 471469..473175(+) (pilB) [Aeromonas veronii strain ANYA 18263]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDDNSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPESELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814515 QBS07_RS02240 WP_279488476.1 471469..473175(+) (pilB) [Aeromonas veronii strain ANYA 18263]
ATGACCTCTAGTCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAAGCCAAAGCTCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTAGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGTGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGACAACAGCCGACTGGACGAGAGCGTCAATACCGCTGATGACGATGCCCCCATCGTCAA
GTACATCAACAAAATCCTGATGGATGCCATCAAGCGTGGTGCCTCCGACCTGCACTTCGAGCCTTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATAGATGGCATTCTGCATGAAATTGCCACACCGCCGGTCAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTCATGGCGCGGCTCGATATTGCCGAACGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGTTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCCCAATACCTGCGTGCCCTCTCCAAACCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGTTCGGGCAAGACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACTAC
CGAGGTAAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGCGTCAATCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACTTTCGCCAGCGCCCTGCGCTCCTTCCTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTTGAAACCGCAGAGATTGCCATCAAGGCGGCGCAGACCGGTCACCTGGTGCTTTCGACCCTGCACACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTACCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGCAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAATCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CGATCGCGCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.415

100

0.565
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.722

95.599

0.542
  pilB Vibrio cholerae strain A1552

54.839

98.239

0.539
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

51.418

99.296

0.511
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

39.241

97.359

0.382
  pilF Thermus thermophilus HB27

40.53

92.958

0.377