Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS23_RS18795 Genome accession   NZ_CP121833
Coordinates   3992520..3994226 (-) Length   568 a.a.
NCBI ID   WP_279500696.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18644     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3987520..3999226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS23_RS18765 mutT 3988117..3988521 (+) 405 WP_204384572.1 8-oxo-dGTP diphosphatase MutT -
  QBS23_RS18770 yacG 3988599..3988793 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS23_RS18775 zapD 3988803..3989524 (-) 722 Protein_3609 cell division protein ZapD -
  QBS23_RS18780 coaE 3989562..3990176 (-) 615 WP_279500693.1 dephospho-CoA kinase -
  QBS23_RS18785 pilD 3990190..3991062 (-) 873 WP_279500694.1 A24 family peptidase Machinery gene
  QBS23_RS18790 pilC 3991124..3992365 (-) 1242 WP_279500695.1 type II secretion system F family protein Machinery gene
  QBS23_RS18795 pilB 3992520..3994226 (-) 1707 WP_279500696.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS23_RS18800 - 3994226..3994645 (-) 420 WP_279500697.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS23_RS18805 nadC 3994961..3995836 (-) 876 WP_216960555.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS23_RS18810 - 3995840..3996310 (-) 471 WP_040068932.1 retropepsin-like aspartic protease -
  QBS23_RS18815 ampD 3996441..3997016 (+) 576 WP_279500698.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS23_RS18820 pdhR 3997295..3998059 (+) 765 WP_058053405.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62591.91 Da        Isoelectric Point: 5.2237

>NTDB_id=814479 QBS23_RS18795 WP_279500696.1 3992520..3994226(-) (pilB) [Aeromonas veronii strain ANYA 18644]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKEPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814479 QBS23_RS18795 WP_279500696.1 3992520..3994226(-) (pilB) [Aeromonas veronii strain ANYA 18644]
ATGACCTCTAGTCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTCACCTTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTACATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAAAACAGCCGCCTCGACGAAAGCGTTAATACCGCCGATGACGATGCTCCAATCGTCAA
ATACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGTGCCTCCGACCTGCACTTCGAGCCTTACGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTCTGCATGAGATCGCCACACCGCCGGTCAACCTCTCCAACCGCTTTTCCGCTCGC
CTCAAGGTGATGGCGCGACTCGATATCGCCGAGCGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAACTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTTACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATACGGGAT
CTGGAAACCGCTGAGATCGCCATCAAGGCGGCCCAGACCGGTCACCTGGTGCTGTCGACCCTGCACACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTCCCCGCCTTCAACATCGCCTCTTCGGTCACCCTGATCATGGCCCAGC
GGCTGGCCCGCAAACTGTGTGACCACTGCAAAGAGCCGGAGGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGTTGGCGGCGGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGAGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATTATGCAGGGGGCAAACTCCCTGCAAATCGCGG
CAATTGCCCAGAAGGAGGGGATGCGTACCCTGCGTACCTCGGGGCTGGAGAAAGCCCGACTAGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.458

95.423

0.539
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377