Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS33_RS01715 Genome accession   NZ_CP121816
Coordinates   353333..355039 (+) Length   568 a.a.
NCBI ID   WP_139411606.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 19953     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 348333..360039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS33_RS01690 pdhR 349480..350244 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QBS33_RS01695 ampD 350523..351098 (-) 576 WP_279496992.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS33_RS01700 - 351229..351699 (+) 471 WP_005336129.1 retropepsin-like aspartic protease -
  QBS33_RS01705 nadC 351703..352572 (+) 870 WP_279496994.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS33_RS01710 - 352931..353128 (+) 198 WP_279497724.1 hypothetical protein -
  QBS33_RS01715 pilB 353333..355039 (+) 1707 WP_139411606.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS33_RS01720 pilC 355280..356521 (+) 1242 WP_279496995.1 type II secretion system F family protein Machinery gene
  QBS33_RS01725 pilD 356583..357455 (+) 873 WP_204382262.1 A24 family peptidase Machinery gene
  QBS33_RS01730 coaE 357469..358083 (+) 615 WP_279496996.1 dephospho-CoA kinase -
  QBS33_RS01735 zapD 358121..358843 (+) 723 WP_005336141.1 cell division protein ZapD -
  QBS33_RS01740 yacG 358853..359047 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QBS33_RS01745 mutT 359126..359530 (-) 405 WP_279496997.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62560.90 Da        Isoelectric Point: 5.3192

>NTDB_id=814382 QBS33_RS01715 WP_139411606.1 353333..355039(+) (pilB) [Aeromonas veronii strain ANYA 19953]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENNRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814382 QBS33_RS01715 WP_139411606.1 353333..355039(+) (pilB) [Aeromonas veronii strain ANYA 19953]
ATGACTTCATCACCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATTAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCTCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGTGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AACTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCTGATCTCGAAGTCTCCGACGAAAACAACCGGCTGGACGAGAGCGTCAATACTGCCGATGATGATGCCCCCATCGTCAA
GTACATCAACAAAATCCTGATGGATGCCATCAAGCGTGGTGCCTCCGACCTGCACTTCGAGCCATATGAGTTCAAGTATC
GGGTTCGCTTTCGCATCGATGGCATTCTGCATGAAATTGCAACCCCGCCGGTTAACCTCTCCAACCGCTTCTCCGCCCGT
CTCAAGGTGATGGCACGACTCGATATTGCCGAACGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCACGCAA
CAAGTCGATGGATATGCGGGTTAATACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACTGGCCCCACCGGCTCGGGTAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTACGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCGGCACAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
AGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGTTGGCGGCAGGGCTTAGACTCTTCAAACCGGTGGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGACGGGT
CGGCATCTACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGCGCAAACTCCCTGCAAATCGCCG
CTATCGCACAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGGCTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACAGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

51.332

99.12

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377