Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS22_RS12435 Genome accession   NZ_CP121801
Coordinates   2628794..2629933 (-) Length   379 a.a.
NCBI ID   WP_026141098.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BOND 19077     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2623794..2634933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS22_RS12415 feoB 2624025..2626298 (+) 2274 WP_279484143.1 Fe(2+) transporter permease subunit FeoB -
  QBS22_RS12420 - 2626295..2626537 (+) 243 WP_279484144.1 FeoC-like transcriptional regulator -
  QBS22_RS12425 - 2626649..2627128 (-) 480 WP_279484145.1 WbuC family cupin fold metalloprotein -
  QBS22_RS12430 glgC 2627264..2628535 (-) 1272 WP_252600990.1 glucose-1-phosphate adenylyltransferase -
  QBS22_RS12435 pilU 2628794..2629933 (-) 1140 WP_026141098.1 type IVa pilus ATPase TapW Machinery gene
  QBS22_RS12440 - 2629993..2630349 (-) 357 WP_005302630.1 DMT family protein -
  QBS22_RS12445 - 2630463..2631194 (+) 732 WP_024942579.1 transporter substrate-binding domain-containing protein -
  QBS22_RS12450 btuC 2631294..2632301 (+) 1008 WP_242763491.1 vitamin B12 ABC transporter permease BtuC -
  QBS22_RS12455 - 2632288..2633073 (+) 786 WP_211049889.1 ATP-binding cassette domain-containing protein -
  QBS22_RS12460 - 2633153..2634214 (-) 1062 WP_017766699.1 3-deoxy-7-phosphoheptulonate synthase -
  QBS22_RS12465 - 2634371..2634751 (+) 381 WP_005302618.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42185.21 Da        Isoelectric Point: 6.2566

>NTDB_id=814344 QBS22_RS12435 WP_026141098.1 2628794..2629933(-) (pilU) [Aeromonas dhakensis strain BOND 19077]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=814344 QBS22_RS12435 WP_026141098.1 2628794..2629933(-) (pilU) [Aeromonas dhakensis strain BOND 19077]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATTTGAAACTGCCCACCGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGTT
CGGGCAAATCCACCTCGCTCGCAGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCGGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCTGCGGTGGAGATCATGCTGGGTACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGAGGAGGAGGAGGCGGTGAAGAACGCCGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAAATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAACCCACCAAGGACG
AGAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

89.446

0.369
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361