Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS22_RS06760 Genome accession   NZ_CP121801
Coordinates   1413513..1415228 (-) Length   571 a.a.
NCBI ID   WP_279485524.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BOND 19077     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1408513..1420228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS22_RS06730 mutT 1409188..1409601 (+) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -
  QBS22_RS06735 yacG 1409663..1409857 (-) 195 WP_042049842.1 DNA gyrase inhibitor YacG -
  QBS22_RS06740 zapD 1409867..1410589 (-) 723 WP_025202564.1 cell division protein ZapD -
  QBS22_RS06745 coaE 1410627..1411241 (-) 615 WP_017766418.1 dephospho-CoA kinase -
  QBS22_RS06750 pilD 1411261..1412133 (-) 873 WP_017779053.1 A24 family peptidase Machinery gene
  QBS22_RS06755 pilC 1412156..1413397 (-) 1242 WP_216988919.1 type II secretion system F family protein Machinery gene
  QBS22_RS06760 pilB 1413513..1415228 (-) 1716 WP_279485524.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS22_RS06765 - 1415219..1415659 (-) 441 WP_010635961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QBS22_RS06770 nadC 1415981..1416838 (-) 858 WP_010635962.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS22_RS06775 - 1416843..1417313 (-) 471 WP_017779055.1 retropepsin-like aspartic protease -
  QBS22_RS06780 ampD 1417437..1418009 (+) 573 WP_279485217.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS22_RS06785 pdhR 1418285..1419049 (+) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62866.37 Da        Isoelectric Point: 5.4342

>NTDB_id=814336 QBS22_RS06760 WP_279485524.1 1413513..1415228(-) (pilB) [Aeromonas dhakensis strain BOND 19077]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMENDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=814336 QBS22_RS06760 WP_279485524.1 1413513..1415228(-) (pilB) [Aeromonas dhakensis strain BOND 19077]
CTGCCCATAATGACATCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCGCAGCGCAAACCCTTTGTGACCTTTCTGATCGAGAACGAGATCCTCGACAGCA
AGGCGCTGGCCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGACACACCCTCTATATCGC
CATGTCCGACCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCG
AAGAGAACAAGCTGACCGCCGCCATCAGCAAGCTGATGGAGAACGATCAGGACGCCCTCGGCATGGAGGACATCGACGAG
TCCGAGATCTCCGAGCTGGAGGTGTCTGACGAAGGGTCACGACTCGACGAGAGCGTCAACACTGCGGATGATGATGCCCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGA
CCAAGTACCGTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGACTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAGCT
GTCGCGCAACAAGTCGATGGACATGCGGGTCAACACCCTGCCTACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCG
ACTCTTCCGCTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCGCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCT
CAACACCACAGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAAATCAACCTGCCTGGGGTCAATCAGGTGCAGGTCA
ACCCCAAGGCTGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTTCTGCGCCAGGATCCCGATGTGGTGATGGTCGGGGAA
ATACGGGATCTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAA
CTCGGCGGCCGAGACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAACATCGCCTCTTCGGTTACCCTGATCA
TGGCTCAGCGACTGGCACGCAAGCTGTGTGACCACTGCAAGGCCCCCGAAGTGGTGCCAGAGGCCGAACTGCTGGAGTTG
GGGTTCACCCAACAGCAACTGGCCGCAGGCTTGCGGCTGTTCAAGCCGGTTGGTTGCAAGGAGTGTTCGGGCGGCTACAA
GGGGCGGGTCGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGC
AGATTGCGGCTATCGCCCAGAAGGAGGGCATGCGCACCTTGCGTACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACC
AGCCTGGCCGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.974

100

0.552
  pilB Acinetobacter baumannii D1279779

54.704

100

0.55
  pilB Legionella pneumophila strain ERS1305867

54.417

99.124

0.539
  pilB Vibrio cholerae strain A1552

53.982

98.949

0.534
  pilB Vibrio campbellii strain DS40M4

51.767

99.124

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.424

0.503
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

96.848

0.375
  pilF Thermus thermophilus HB27

40.53

92.469

0.375