Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBS35_RS18715 Genome accession   NZ_CP121800
Coordinates   4075482..4077188 (+) Length   568 a.a.
NCBI ID   WP_042049838.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain DAMIAN 19342     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4070482..4082188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS35_RS18690 pdhR 4071663..4072427 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QBS35_RS18695 ampD 4072703..4073275 (-) 573 WP_279464500.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QBS35_RS18700 - 4073399..4073869 (+) 471 WP_043171614.1 retropepsin-like aspartic protease -
  QBS35_RS18705 nadC 4073874..4074731 (+) 858 WP_043171615.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QBS35_RS18710 tapA 4075053..4075475 (+) 423 WP_043171616.1 type IVa pilus major pilin TapA -
  QBS35_RS18715 pilB 4075482..4077188 (+) 1707 WP_042049838.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QBS35_RS18720 pilC 4077304..4078545 (+) 1242 WP_201879871.1 type II secretion system F family protein Machinery gene
  QBS35_RS18725 pilD 4078568..4079440 (+) 873 WP_201879868.1 A24 family peptidase Machinery gene
  QBS35_RS18730 coaE 4079460..4080074 (+) 615 WP_201879854.1 dephospho-CoA kinase -
  QBS35_RS18735 zapD 4080112..4080834 (+) 723 WP_026141001.1 cell division protein ZapD -
  QBS35_RS18740 yacG 4080844..4081038 (+) 195 WP_201879851.1 DNA gyrase inhibitor YacG -
  QBS35_RS18745 mutT 4081099..4081512 (-) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62515.91 Da        Isoelectric Point: 5.4342

>NTDB_id=814315 QBS35_RS18715 WP_042049838.1 4075482..4077188(+) (pilB) [Aeromonas dhakensis strain DAMIAN 19342]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=814315 QBS35_RS18715 WP_042049838.1 4075482..4077188(+) (pilB) [Aeromonas dhakensis strain DAMIAN 19342]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCAGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
CCCGACCAATGTCTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGCTGCACACCGAGGCCCTGCTGGTCGAGGAGAACA
AGCTGACCGCCGCCATCAGCAAGCTGATGGAGAGCGATCAGGACGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAGGTGTCTGACGAAGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAACCCTATGAGACCAAGTACC
GTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGACGGCTACCCCAGGATGGCCGCATCAAATTGAAAATGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAACACCCTGCCTACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCGACTCTTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCGCTTTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGTCCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATCCTCAATACCAC
AGAGGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCAGGAGTCAATCAGGTGCAGGTCAACCCCAAGG
CTGGACTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTGGTGATGGTTGGTGAAATACGGGAT
CTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACATTGCACACCAACTCGGCGGC
CGAGACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTTAACATCGCCTCCTCGGTAACCCTGATCATGGCTCAGC
GACTGGCACGCAAGCTGTGCGACCACTGCAAGGCCCCCGAGGTGGTGCCGGAAGCTGAACTGCTGGAGTTGGGGTTCACC
CAACAGCAACTGGCCGCAGGCTTGCGGCTGTTCAAGCCGGTTGGTTGCAAGGAGTGTTCGGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGCAGATTGCGG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGGACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACCAGCCTGGCC
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Acinetobacter baumannii D1279779

54.833

100

0.549
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.045

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.944

0.504
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377
  pilF Thermus thermophilus HB27

40.53

92.958

0.377