Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS05_RS17905 Genome accession   NZ_CP121795
Coordinates   3864461..3865600 (+) Length   379 a.a.
NCBI ID   WP_017766703.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain DAMIAN 12594     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3859461..3870600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS05_RS17875 - 3859652..3860032 (-) 381 WP_005302618.1 diacylglycerol kinase -
  QBS05_RS17880 - 3860189..3861250 (+) 1062 WP_005302620.1 3-deoxy-7-phosphoheptulonate synthase -
  QBS05_RS17885 - 3861330..3862115 (-) 786 WP_279461716.1 ATP-binding cassette domain-containing protein -
  QBS05_RS17890 btuC 3862102..3863109 (-) 1008 WP_279461717.1 vitamin B12 ABC transporter permease BtuC -
  QBS05_RS17895 - 3863209..3863931 (-) 723 WP_279461719.1 transporter substrate-binding domain-containing protein -
  QBS05_RS17900 - 3864045..3864401 (+) 357 WP_005302630.1 DMT family protein -
  QBS05_RS17905 pilU 3864461..3865600 (+) 1140 WP_017766703.1 type IVa pilus ATPase TapW Machinery gene
  QBS05_RS17910 glgC 3865883..3867154 (+) 1272 WP_005302634.1 glucose-1-phosphate adenylyltransferase -
  QBS05_RS17915 - 3867290..3867769 (+) 480 WP_010633537.1 WbuC family cupin fold metalloprotein -
  QBS05_RS17920 - 3867892..3868134 (-) 243 WP_279461721.1 FeoC-like transcriptional regulator -
  QBS05_RS17925 feoB 3868131..3870404 (-) 2274 WP_042006621.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42171.19 Da        Isoelectric Point: 6.2561

>NTDB_id=814266 QBS05_RS17905 WP_017766703.1 3864461..3865600(+) (pilU) [Aeromonas dhakensis strain DAMIAN 12594]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=814266 QBS05_RS17905 WP_017766703.1 3864461..3865600(+) (pilU) [Aeromonas dhakensis strain DAMIAN 12594]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGACTTGAAACTGCCCACCGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGCT
CGGGCAAATCCACCTCGCTCGCGGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCCTTCTCGGAGACCGGCCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCCGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGGTCA
AACGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTCGATCTGGTGGTCGAAGGGGTGATCGACGAGGAGGAAGCGGTGAAGAACGCCGATTCGGCCAACAATCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAACCCACCAAGGACG
AGAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361