Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBJ73_RS08780 Genome accession   NZ_CP121776
Coordinates   1847828..1849540 (-) Length   570 a.a.
NCBI ID   WP_308469424.1    Uniprot ID   A0AAJ6IB87
Organism   Acinetobacter johnsonii strain AYTCM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1842828..1854540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBJ73_RS08750 (QBJ73_08755) - 1842925..1843248 (+) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -
  QBJ73_RS08755 (QBJ73_08760) rlmB 1843391..1844140 (+) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  QBJ73_RS08760 (QBJ73_08765) - 1844173..1845090 (+) 918 WP_029574926.1 DMT family transporter -
  QBJ73_RS08765 (QBJ73_08770) coaE 1845105..1845704 (-) 600 WP_010325765.1 dephospho-CoA kinase -
  QBJ73_RS08770 (QBJ73_08775) pilD 1845711..1846571 (-) 861 WP_010325766.1 A24 family peptidase Machinery gene
  QBJ73_RS08775 (QBJ73_08780) pilC 1846571..1847797 (-) 1227 WP_010325767.1 type II secretion system F family protein Machinery gene
  QBJ73_RS08780 (QBJ73_08785) pilB 1847828..1849540 (-) 1713 WP_308469424.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QBJ73_RS08785 (QBJ73_08790) tpiA 1849820..1850611 (+) 792 WP_010325769.1 triose-phosphate isomerase -
  QBJ73_RS08790 (QBJ73_08795) secG 1850625..1850954 (+) 330 WP_004692710.1 preprotein translocase subunit SecG -
  QBJ73_RS08820 (QBJ73_08825) rimP 1851909..1852433 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  QBJ73_RS08825 (QBJ73_08830) nusA 1852473..1853957 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63534.83 Da        Isoelectric Point: 4.9477

>NTDB_id=814174 QBJ73_RS08780 WP_308469424.1 1847828..1849540(-) (pilB) [Acinetobacter johnsonii strain AYTCM]
MSVSNGLPKFTGFIRRLVEDGRVTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKASVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNLVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=814174 QBJ73_RS08780 WP_308469424.1 1847828..1849540(-) (pilB) [Acinetobacter johnsonii strain AYTCM]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAACCATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATTCTCTATAT
CGCTACCAGCAATCCAACCAACATGGATGCGATGGACGCGATTCGCTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCCCAGCTTGAAAAACTGATCGAACAAAATTTCGCGCAAGCTGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCGCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGTGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGTGTAGATGGAGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGCCTCGCCTCT
CGTTTAAAAGTCATGTCACAAATGGACATTTCTGAAAAGCGCGTGCCACAAGATGGCCGAATTAAATTAAAGCTGTCAAA
AAGTAAAGCCATCGACTTTCGTGTCAACTCACTTCCAACCTTGTTTGGCGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGACCAAAAAGATCTGTTTATGCAGGCTTTAGACAAACCT
CAAGGCATGTTGCTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCTCTATATACAGGTCTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCGGAAGATCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAACGTGAACAATA
AAGTCGGCCTTACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAGGATCCTGATATTGTGATGGTCGGTGAGATTCGT
GACCTTGAAACAGCTGAAATCGCGATTAAAGCCGCGCAGACAGGCCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGCAATATGGGGGTTCCCTCTTTTAACATTGCGACCTCGGTCAATTTGGTAATTGCAC
AACGTTTAGCACGCCGCTTATGTAGCCAATGCAAAGCCTCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCTTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.667

100

0.767
  pilB Legionella pneumophila strain ERS1305867

53.745

93.684

0.504
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.148

99.474

0.479
  pilB Vibrio campbellii strain DS40M4

47.67

97.895

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

50.484

90.702

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

34.466

100

0.374
  pilF Thermus thermophilus HB27

36.602

100

0.367