Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBL01_RS04145 Genome accession   NZ_CP121769
Coordinates   792111..793496 (+) Length   461 a.a.
NCBI ID   WP_279378642.1    Uniprot ID   A0AAJ6AKB8
Organism   Glaesserella parasuis strain Z44     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 787111..798496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBL01_RS04120 (QBL01_04120) gpt 787547..788026 (+) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  QBL01_RS04125 (QBL01_04125) gmk 788280..788906 (+) 627 WP_075606467.1 guanylate kinase -
  QBL01_RS04130 (QBL01_04130) radA 788959..790335 (-) 1377 WP_261482606.1 DNA repair protein RadA -
  QBL01_RS04135 (QBL01_04135) pilA 790523..790969 (+) 447 WP_160442335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QBL01_RS04140 (QBL01_04140) - 791310..791675 (+) 366 WP_160433175.1 DUF559 domain-containing protein -
  QBL01_RS04145 (QBL01_04145) pilB 792111..793496 (+) 1386 WP_279378642.1 GspE/PulE family protein Machinery gene
  QBL01_RS04150 (QBL01_04150) pilC 793489..794685 (+) 1197 WP_279378643.1 type II secretion system F family protein Machinery gene
  QBL01_RS04155 (QBL01_04155) - 794682..795350 (+) 669 WP_021117769.1 prepilin peptidase -
  QBL01_RS04160 (QBL01_04160) dapE 795434..796567 (+) 1134 WP_021117768.1 succinyl-diaminopimelate desuccinylase -
  QBL01_RS04165 (QBL01_04165) clpX 796641..797891 (-) 1251 WP_042906659.1 ATP-dependent protease ATP-binding subunit ClpX -
  QBL01_RS04170 (QBL01_04170) clpP 797898..798479 (-) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52659.41 Da        Isoelectric Point: 5.1464

>NTDB_id=814109 QBL01_RS04145 WP_279378642.1 792111..793496(+) (pilB) [Glaesserella parasuis strain Z44]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQHPLYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLHKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALLAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGTGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=814109 QBL01_RS04145 WP_279378642.1 792111..793496(+) (pilB) [Glaesserella parasuis strain Z44]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAACCTGTGGTAATTGCTTCTGATGAG
CTGAAATACCTGCTTAACGCACTCTCGCCTGAACAACACCCCCTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAGCCCAAAATGCGT
CTGATATTCATATTGAACCACGCAAGCAAAAATTGATTATTCGTCTACGCATTGACGGTGTTTTGCACCTGCATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCTTGTATAGTGCCTTAAACTACCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCTGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTATTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATTAGCGGTCGAACGTTTGTTACAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCACAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGTAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTAGATTTTTCCAGCTTACGAGAGAGTGCTAAACAAAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-93)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

96.529

100

0.965
  pilB Haemophilus influenzae Rd KW20

57.363

98.698

0.566
  pilB Haemophilus influenzae 86-028NP

56.739

99.783

0.566
  pilB Vibrio cholerae strain A1552

38.755

100

0.419
  pilB Vibrio parahaemolyticus RIMD 2210633

39.501

100

0.412
  pilB Vibrio campbellii strain DS40M4

39.501

100

0.412
  pilB Acinetobacter baylyi ADP1

36.832

100

0.403
  pilB Acinetobacter baumannii D1279779

36.187

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.298

100

0.39
  pilB Legionella pneumophila strain ERS1305867

40.455

95.445

0.386
  pilF Neisseria gonorrhoeae MS11

36.842

100

0.38