Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QBL01_RS04135 Genome accession   NZ_CP121769
Coordinates   790523..790969 (+) Length   148 a.a.
NCBI ID   WP_160442335.1    Uniprot ID   K4JZ63
Organism   Glaesserella parasuis strain Z44     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 785523..795969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBL01_RS04115 (QBL01_04115) - 785913..787367 (-) 1455 WP_279378641.1 aminoacyl-histidine dipeptidase -
  QBL01_RS04120 (QBL01_04120) gpt 787547..788026 (+) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  QBL01_RS04125 (QBL01_04125) gmk 788280..788906 (+) 627 WP_075606467.1 guanylate kinase -
  QBL01_RS04130 (QBL01_04130) radA 788959..790335 (-) 1377 WP_261482606.1 DNA repair protein RadA -
  QBL01_RS04135 (QBL01_04135) pilA 790523..790969 (+) 447 WP_160442335.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QBL01_RS04140 (QBL01_04140) - 791310..791675 (+) 366 WP_160433175.1 DUF559 domain-containing protein -
  QBL01_RS04145 (QBL01_04145) pilB 792111..793496 (+) 1386 WP_279378642.1 GspE/PulE family protein Machinery gene
  QBL01_RS04150 (QBL01_04150) pilC 793489..794685 (+) 1197 WP_279378643.1 type II secretion system F family protein Machinery gene
  QBL01_RS04155 (QBL01_04155) - 794682..795350 (+) 669 WP_021117769.1 prepilin peptidase -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15568.99 Da        Isoelectric Point: 8.7869

>NTDB_id=814108 QBL01_RS04135 WP_160442335.1 790523..790969(+) (pilA) [Glaesserella parasuis strain Z44]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNTGALTNCTGGSNGV
QANKTDVADAKYLKSISVAAGVITVAGKGNIDGYGYTMTPTLANNNITWTTSCRGSDTSLFPANFCGQ

Nucleotide


Download         Length: 447 bp        

>NTDB_id=814108 QBL01_RS04135 WP_160442335.1 790523..790969(+) (pilA) [Glaesserella parasuis strain Z44]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTGATGATCGTGATCGCCATCATTGC
TATTTTAGCCACGATTGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCTGCACTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACACAGGGGCTTTAACTAACTGTACAGGGGGTTCTAACGGCGTT
CAAGCCAATAAAACCGATGTTGCCGATGCTAAATATCTTAAATCTATTTCCGTCGCTGCAGGTGTAATCACAGTGGCTGG
TAAAGGCAACATTGATGGCTACGGCTACACAATGACCCCGACATTAGCCAATAACAACATCACTTGGACAACAAGCTGTC
GAGGTTCTGACACCAGTTTATTCCCTGCGAATTTCTGTGGGCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K4JZ63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

86

100

0.872
  pilA Haemophilus influenzae 86-028NP

59.184

99.324

0.588
  pilA Haemophilus influenzae Rd KW20

57.143

99.324

0.568
  comP Acinetobacter baylyi ADP1

45.517

97.973

0.446
  pilE Neisseria gonorrhoeae MS11

36.986

98.649

0.365