Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QBE03_RS07090 Genome accession   NZ_CP121698
Coordinates   1609342..1611075 (+) Length   577 a.a.
NCBI ID   WP_267096240.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain DJ16     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1604342..1616075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBE03_RS07070 coaE 1605571..1606200 (-) 630 WP_279363091.1 dephospho-CoA kinase -
  QBE03_RS07075 - 1606214..1607077 (-) 864 WP_017910397.1 A24 family peptidase -
  QBE03_RS07080 pilC 1607084..1608340 (-) 1257 WP_267096242.1 type II secretion system F family protein Machinery gene
  QBE03_RS07085 - 1608723..1609163 (+) 441 WP_267096241.1 pilin -
  QBE03_RS07090 pilB 1609342..1611075 (+) 1734 WP_267096240.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QBE03_RS07095 pilR 1611241..1612629 (-) 1389 WP_279363097.1 sigma-54 dependent transcriptional regulator Regulator
  QBE03_RS07100 - 1612712..1614325 (-) 1614 WP_267096239.1 ATP-binding protein -
  QBE03_RS07105 sucC 1614716..1615885 (+) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62599.86 Da        Isoelectric Point: 5.6425

>NTDB_id=813999 QBE03_RS07090 WP_267096240.1 1609342..1611075(+) (pilB) [Xanthomonas sacchari strain DJ16]
MNATAAPNLVGITGIARRLVQDGALDEAAARTAMAQAGEAKVPLPQWFSEKKLVTAAQLAAANAMEFGMPLMDVSVFDAS
QNAMKLVSEELLQKYQVLPLFKRGGRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGSALG
DDEGMDNLDVGGGDEDMGGGGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLPE
KALLAEGFTEAEIAAGVQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVKDLRQSALLK
ARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=813999 QBE03_RS07090 WP_267096240.1 1609342..1611075(+) (pilB) [Xanthomonas sacchari strain DJ16]
ATGAATGCTACTGCAGCGCCGAACCTTGTCGGCATCACCGGTATCGCCCGCCGCCTCGTGCAGGACGGCGCGCTGGACGA
AGCCGCGGCGCGGACCGCGATGGCCCAGGCGGGCGAGGCCAAGGTGCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACCGCCGCGCAGCTGGCCGCCGCCAATGCGATGGAGTTCGGCATGCCGTTGATGGATGTTTCGGTCTTCGATGCCAGC
CAGAACGCGATGAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCGGCCGCCT
GTTCGTCGGCATCAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGCCGGACCCTGGAGCAGTGGCAGGCCAGCAACGATTCCCTCGGCAGCGCCTTGGGC
GACGACGAGGGCATGGACAACCTCGATGTCGGCGGCGGCGACGAGGACATGGGCGGCGGTGGCGACACCGGCATCGACGC
CAAGGGGGAGGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTGGATGCGATCCGCCGCGGCGCGTCCGACATCC
ACTTCGAGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAAGGCGCCGGTC
AAGCTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGGGTCCCGCAGGACGG
CCGCATCAAGCTCAATCTGTCCAAGAACAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACGCTGTTCGGCGAGAAGA
TCGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGTATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAGCTG
TTCCTGGACGCGATCCACAAGCCGTACGGCATGGTCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTA
CACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCCTGCCCGGGG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTTGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACCCTGCACACCAACGACGCGCCGCAGACCATTGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCA
GTTCGGTGACCCTGGTCATCGCCCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCCCGGTCCAGTTGCCGGAG
AAGGCCTTGCTGGCCGAAGGCTTCACCGAGGCGGAGATCGCCGCCGGCGTCCAACTGTACGAAGCGGTCGGCTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTACCAAGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGCTGC
AGGGCGGCAACGCCATGGACATCGCCAGTGCCGCGCAGAAGATCGGCGTCAAGGACCTGCGCCAGTCGGCCCTGCTCAAG
GCCCGCAACGGCATCACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.671

100

0.548
  pilB Acinetobacter baumannii D1279779

54.912

98.787

0.542
  pilB Legionella pneumophila strain ERS1305867

51.142

98.614

0.504
  pilB Vibrio cholerae strain A1552

49.913

99.307

0.496
  pilF Neisseria gonorrhoeae MS11

49.296

98.44

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

46.046

98.614

0.454
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

36.557

100

0.386
  pilF Thermus thermophilus HB27

39.619

90.988

0.36