Detailed information NTG0812858

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QA712_RS05200
• Genome accession: NZ_CP121539
• Coordinates: 934009..934656 (-)
• Length: 215 a.a.
• NCBI ID: WP_332528945.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain MA5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 929009..939656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QA712_RS05175 (QA712_05160)	atpF	929078..929584 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
QA712_RS05180 (QA712_05165)	atpB	929599..930312 (-)	714	WP_372757834.1	F0F1 ATP synthase subunit A	-
QA712_RS05185 (QA712_05170)	-	930357..930572 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
QA712_RS05190 (QA712_05175)	-	930757..931533 (-)	777	WP_311803157.1	alpha/beta hydrolase family protein	-
QA712_RS05195 (QA712_05180)	comEC	931818..934028 (-)	2211	WP_332528946.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QA712_RS05200 (QA712_05185)	comEA	934009..934656 (-)	648	WP_332528945.1	ComEA family DNA-binding protein	Machinery gene
QA712_RS05205 (QA712_05190)	-	934716..936077 (-)	1362	WP_332528944.1	ABC transporter permease	-
QA712_RS05210 (QA712_05195)	-	936074..937006 (-)	933	WP_372757837.1	ABC transporter ATP-binding protein	-
QA712_RS05215 (QA712_05200)	-	937111..937509 (-)	399	WP_195936199.1	hypothetical protein	-
QA712_RS05220 (QA712_05205)	-	937613..938176 (-)	564	WP_372757839.1	N-acetyltransferase family protein	-
QA712_RS05225 (QA712_05210)	-	938349..939527 (-)	1179	WP_195936200.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23490.67 Da
• Isoelectric Point: 5.1610

>NTDB_id=812858 QA712_RS05200 WP_332528945.1 934009..934656(-) (comEA) [Lactococcus lactis strain MA5]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTGNTDSSANSVISQPSTTTAKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=812858 QA712_RS05200 WP_332528945.1 934009..934656(-) (comEA) [Lactococcus lactis strain MA5]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATACTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGCGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAACCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
GAAATACTGACAGCTCTGCTAATTCGGTCATTAGTCAGCCCTCAACAACCACTGCAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (152-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	74.312	100	0.753
comEA/celA/cilE	Streptococcus mitis SK321	44.037	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	42.661	100	0.433
comEA/celA/cilE	Streptococcus pneumoniae R6	41.743	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.743	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.743	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	40.826	100	0.414
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381
comEA	Staphylococcus aureus MW2	37.559	99.07	0.372
comEA	Staphylococcus aureus N315	37.089	99.07	0.367