Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   QA712_RS01885 Genome accession   NZ_CP121539
Coordinates   264701..265549 (-) Length   282 a.a.
NCBI ID   WP_012897825.1    Uniprot ID   A0A2A9I6Y4
Organism   Lactococcus lactis strain MA5     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 259701..270549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QA712_RS01870 (QA712_01850) xerS 259775..260845 (+) 1071 WP_372757433.1 tyrosine recombinase XerS -
  QA712_RS01875 (QA712_01855) trmFO 260892..262235 (-) 1344 WP_311803373.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  QA712_RS01880 (QA712_01860) topA 262307..264439 (-) 2133 WP_003131148.1 type I DNA topoisomerase -
  QA712_RS01885 (QA712_01865) dprA 264701..265549 (-) 849 WP_012897825.1 DNA-processing protein DprA Machinery gene
  QA712_RS01890 (QA712_01870) - 265695..266075 (-) 381 WP_047206180.1 RidA family protein -
  QA712_RS01895 (QA712_01875) budA 266116..266826 (-) 711 WP_332529287.1 acetolactate decarboxylase -
  QA712_RS01900 (QA712_01880) ilvA 266942..268192 (-) 1251 WP_047206181.1 threonine ammonia-lyase IlvA -
  QA712_RS01905 (QA712_01885) ilvC 268248..269270 (-) 1023 WP_017864494.1 ketol-acid reductoisomerase -
  QA712_RS01910 (QA712_01890) ilvN 269316..269792 (-) 477 WP_010905839.1 acetolactate synthase small subunit -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 32119.17 Da        Isoelectric Point: 9.5086

>NTDB_id=812846 QA712_RS01885 WP_012897825.1 264701..265549(-) (dprA) [Lactococcus lactis strain MA5]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDVSKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide


Download         Length: 849 bp        

>NTDB_id=812846 QA712_RS01885 WP_012897825.1 264701..265549(-) (dprA) [Lactococcus lactis strain MA5]
ATGATAACAAATTTTGATTTATATCGCTGGAAAAAAGCTGGCATGACCAATTTAGGAGTGAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCTAACTTTATTGAAC
AATATAAAAATCAAGACGTCAGTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGGCTCAAGGAAATTTACAATCCACCGGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTAGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAATATCTTGCTAAAAACCA
ACTAATTCTCTCAGAATATTCAGTCGGAGAAAAACCACTTCGCTATCATTTTCCTGAACGCAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTGCGAAGCGGTAGTTTAATTACTTGCGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATTCAACAAGGAGCAAA
ATTAATCTACCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)

DNA recombination-mediator protein A (DNA_processg_A)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A9I6Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

86.879

100

0.869
  dprA Streptococcus mutans UA159

60.072

98.582

0.592
  dprA/cilB/dalA Streptococcus mitis SK321

54.643

99.291

0.543
  dprA/cilB/dalA Streptococcus pneumoniae R6

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae D39

53.929

99.291

0.535
  dprA Legionella pneumophila strain ERS1305867

39.13

97.872

0.383
  dprA Haemophilus influenzae Rd KW20

38.889

95.745

0.372
  dprA Bacillus subtilis subsp. subtilis str. 168

43.777

82.624

0.362
  dprA Latilactobacillus sakei subsp. sakei 23K

40

90.426

0.362