Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P3S51_RS01565 Genome accession   NZ_CP121303
Coordinates   320954..322666 (-) Length   570 a.a.
NCBI ID   WP_311918164.1    Uniprot ID   -
Organism   Acinetobacter sp. ANC 7201     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315954..327666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3S51_RS01535 (P3S51_01535) - 316082..316405 (+) 324 WP_154772002.1 pyrimidine/purine nucleoside phosphorylase -
  P3S51_RS01540 (P3S51_01540) rlmB 316538..317287 (+) 750 WP_248103377.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  P3S51_RS01545 (P3S51_01545) - 317307..318227 (+) 921 WP_248103375.1 DMT family transporter -
  P3S51_RS01550 (P3S51_01550) coaE 318224..318829 (-) 606 WP_248103370.1 dephospho-CoA kinase -
  P3S51_RS01555 (P3S51_01555) pilD 318832..319698 (-) 867 WP_282441558.1 A24 family peptidase Machinery gene
  P3S51_RS01560 (P3S51_01560) pilC 319698..320924 (-) 1227 WP_154772007.1 type II secretion system F family protein Machinery gene
  P3S51_RS01565 (P3S51_01565) pilB 320954..322666 (-) 1713 WP_311918164.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P3S51_RS01570 (P3S51_01570) tpiA 322943..323734 (+) 792 WP_154772009.1 triose-phosphate isomerase -
  P3S51_RS01575 (P3S51_01575) secG 323748..324077 (+) 330 WP_154772010.1 preprotein translocase subunit SecG -
  P3S51_RS01605 (P3S51_01605) rimP 325005..325529 (+) 525 WP_154772011.1 ribosome maturation factor RimP -
  P3S51_RS01610 (P3S51_01610) nusA 325565..327049 (+) 1485 WP_311918165.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63391.57 Da        Isoelectric Point: 4.7368

>NTDB_id=811737 P3S51_RS01565 WP_311918164.1 320954..322666(-) (pilB) [Acinetobacter sp. ANC 7201]
MSVIQSTPKFTGFIRRLVEDGVVDATSMQNAIINAKKANQDIIPYLTQNLKVSSLTIAETIALEFGEPIFDLSVYNTDML
AKDAVDEKLIIKHRVLPIFNRGNLLYVATSNPTNIEAIDAIRFNSKMNVESVIVEFDKLDKLIENLFADTGNFEFSDDEF
DLDVEEVDPNQENQKDEDPTGDESPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKISKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMDALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKIGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVQSFNIATSVNLVIAQRLARRLCSHCKAPADIPEQSLLELGF
TTEDLADPETKIYQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASEKAGFRNLRRSGLIKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=811737 P3S51_RS01565 WP_311918164.1 320954..322666(-) (pilB) [Acinetobacter sp. ANC 7201]
ATGTCAGTCATACAATCTACGCCGAAATTCACCGGTTTTATTCGACGCCTTGTTGAAGATGGGGTTGTTGATGCTACATC
AATGCAAAATGCCATCATCAATGCAAAAAAAGCCAATCAAGATATCATTCCTTATTTGACCCAAAATTTAAAAGTTTCTT
CACTCACCATTGCTGAGACTATTGCTTTAGAATTTGGTGAACCTATTTTCGACTTATCTGTATACAACACAGATATGCTC
GCAAAAGATGCTGTTGATGAAAAACTCATAATAAAACATCGTGTTTTACCTATTTTTAACCGAGGTAATCTTCTATATGT
GGCAACCAGTAATCCCACAAATATAGAAGCGATAGATGCCATTCGCTTTAACAGTAAAATGAATGTTGAATCTGTTATTG
TCGAGTTTGATAAACTAGATAAACTTATTGAAAATTTATTTGCAGATACAGGGAACTTTGAGTTTAGTGATGATGAGTTC
GATTTAGATGTTGAAGAAGTTGATCCTAATCAAGAAAATCAAAAAGATGAAGACCCAACAGGTGATGAATCTCCAATTGT
AAAATATATCAATAAACTCTTAATTGATGCTATTCGTATGGGCGCTTCAGATTTACACTTTGAACCCTATGAAAAATCTT
ATCGTGTTCGATACCGTGTAGATGGTGTATTACGTCAAATTGCAACTCCCCCACTACAGTTAGCAAACCGTTTAGCATCA
CGTTTAAAAGTAATGTCACAAATGGACATTTCAGAAAAACGTGTTCCGCAAGATGGTCGTATTAAATTAAAAATCTCAAA
AACAAAAGCTATCGACTTCCGTGTTAACTCACTCCCAACCTTATTTGGTGAGAAACTCGTACTTCGTATTTTAGATCCAT
CTAGTGCGATGTTGGGTATTGATGCTTTGGGTTATGAGCCTGATCAAAAAGAACTTTTCATGGATGCACTTGATAAACCT
CAAGGCATGCTATTAATTACAGGTCCTACTGGTTCGGGTAAAACGGTATCTTTATATACAGGTTTGAATATTCTAAACCG
TGAAGATACCAATATTTCAACAGCCGAAGATCCTGTCGAAATTAACTTAGAAGGTATTAACCAAGTTAACGTTAATAATA
AAATTGGTTTAACCTTCTCTGCGGCACTTAAATCATTTCTTCGTCAAGATCCAGATATTGTTATGGTCGGGGAAATTCGT
GACCTTGAAACAGCTGAAATTGCAATTAAAGCAGCACAAACAGGTCATATGGTAATGTCCACACTACATACAAACAGCGC
ACCTGAAACATTAACGCGCTTAAGAAATATGGGCGTTCAATCTTTCAATATTGCAACATCTGTTAACCTTGTTATTGCAC
AACGTTTAGCACGTCGTTTATGCTCCCACTGCAAAGCTCCTGCCGACATTCCAGAACAAAGCTTATTAGAACTAGGATTC
ACTACAGAAGACTTAGCTGATCCAGAAACTAAAATTTATCAACCTGTAGGCTGTTCTGAGTGTCGTGAAGGTTATAAAGG
ACGTGTAGGTATTTATGAGGTAATGAAAGTTACACCAGAAATTTCAAAAATTATTATGGAAGATGGTAATGCACTTCAAA
TTGCAGAAGCCTCTGAAAAAGCAGGTTTTAGAAACTTACGCCGTTCTGGTTTAATAAAAGTCATGCAAGGGGTAACTTCT
TTACAAGAAGTAAATCGTGTGACGAGTGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.825

100

0.798
  pilB Acinetobacter baylyi ADP1

76.708

100

0.768
  pilB Legionella pneumophila strain ERS1305867

51.394

100

0.518
  pilF Neisseria gonorrhoeae MS11

50.712

98.596

0.5
  pilB Vibrio cholerae strain A1552

49.296

99.649

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.91

97.368

0.486
  pilB Vibrio campbellii strain DS40M4

50.672

91.404

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

35.113

100

0.381
  pilF Thermus thermophilus HB27

36.87

100

0.372