Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   P8934_RS10340 Genome accession   NZ_CP121193
Coordinates   2155565..2156797 (+) Length   410 a.a.
NCBI ID   WP_278030958.1    Uniprot ID   -
Organism   Halomonas sp. CKK8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2150565..2161797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8934_RS10310 (P8934_10310) - 2151230..2151529 (+) 300 WP_278030952.1 type II toxin-antitoxin system RelE/ParE family toxin -
  P8934_RS10315 (P8934_10315) - 2151526..2151822 (+) 297 WP_278030953.1 addiction module antidote protein -
  P8934_RS10320 (P8934_10320) - 2151897..2152163 (-) 267 WP_278030954.1 type II toxin-antitoxin system RelE/ParE family toxin -
  P8934_RS10325 (P8934_10325) - 2152147..2152368 (-) 222 WP_278030955.1 TraY domain-containing protein -
  P8934_RS10330 (P8934_10330) - 2152682..2153050 (-) 369 WP_278030956.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P8934_RS10335 (P8934_10335) pilB 2153480..2155276 (+) 1797 WP_278030957.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P8934_RS10340 (P8934_10340) pilC 2155565..2156797 (+) 1233 WP_278030958.1 type II secretion system F family protein Machinery gene
  P8934_RS10345 (P8934_10345) - 2156978..2157496 (-) 519 WP_278030959.1 L,D-transpeptidase -
  P8934_RS10350 (P8934_10350) - 2157496..2158164 (-) 669 WP_278030960.1 TetR/AcrR family transcriptional regulator -
  P8934_RS10355 (P8934_10355) lexA 2158602..2159255 (+) 654 WP_133633777.1 transcriptional repressor LexA -
  P8934_RS10360 (P8934_10360) dinB 2159280..2160338 (+) 1059 WP_278030961.1 DNA polymerase IV -
  P8934_RS10365 (P8934_10365) - 2160422..2161093 (-) 672 WP_278030962.1 energy-coupling factor ABC transporter permease -
  P8934_RS10370 (P8934_10370) - 2161361..2161543 (+) 183 WP_278030963.1 type II toxin-antitoxin system HicA family toxin -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45319.19 Da        Isoelectric Point: 10.2756

>NTDB_id=810899 P8934_RS10340 WP_278030958.1 2155565..2156797(+) (pilC) [Halomonas sp. CKK8]
MATKPMRKHQKVKLYRWRWTGMGPNGRKVAGEIVATKRVEVETILAGQNIVAKRIRRKSGIGSGMGTIKPRDIMLFARQM
ATMIRAGVPVLQAFQVVAESLKKPAMSALVQELMNDVAAGASFSEALRRHPQHFDRLFANMVEAGEQSGSLDRMLERVAA
YKEKMESLKSRVKKALWYPAAVISVGVGVTALLLIKVVPQFESLFHGFGAELPAPTRITIALSEFAQQYWWWGVLGLVAF
IFFVRTAMKRSEAFRYRMHHLALRIPVIGDILDKSSVARYSRTLATTFGAGVPLVEALETAQGAAGNLVYERAIAQIRED
VSSGQQLNFAMRMTEKFPALAVQMVGIGEEAGSLDAMLNRVADYYEEEVDNKVDTLTSLLEPFIIVVLGVLVGGLVISMY
LPIFELGSVI

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=810899 P8934_RS10340 WP_278030958.1 2155565..2156797(+) (pilC) [Halomonas sp. CKK8]
ATGGCAACGAAGCCCATGCGCAAGCACCAGAAGGTGAAGCTCTATCGCTGGCGCTGGACGGGCATGGGACCCAATGGTCG
CAAGGTCGCCGGCGAGATCGTCGCCACGAAGCGGGTGGAGGTGGAAACCATCCTCGCCGGCCAGAACATCGTGGCCAAGC
GGATCCGCCGCAAGAGCGGCATCGGCAGCGGCATGGGCACGATCAAGCCGCGGGACATCATGCTCTTCGCCCGCCAGATG
GCGACCATGATCCGCGCCGGGGTGCCGGTGCTGCAGGCCTTCCAGGTGGTGGCCGAGAGCCTCAAGAAGCCGGCGATGAG
CGCCCTGGTGCAGGAGCTGATGAACGACGTGGCGGCCGGCGCCAGCTTCTCCGAGGCCCTCAGGCGCCATCCCCAGCACT
TCGACCGGCTGTTCGCCAACATGGTGGAGGCCGGGGAGCAGTCCGGCTCGCTGGACCGCATGCTCGAGCGCGTGGCGGCC
TACAAGGAGAAGATGGAGTCGCTCAAGAGCCGGGTGAAGAAGGCGCTCTGGTACCCGGCGGCGGTGATCTCGGTGGGCGT
CGGCGTGACGGCCCTGCTGCTGATCAAGGTGGTGCCGCAGTTCGAGAGCCTCTTCCACGGCTTCGGCGCCGAGCTCCCCG
CCCCGACCCGCATCACCATCGCGCTCTCGGAATTCGCCCAGCAGTACTGGTGGTGGGGGGTGCTGGGCCTGGTGGCCTTC
ATCTTCTTTGTACGCACTGCCATGAAGCGCTCCGAGGCCTTTCGCTACCGCATGCATCACCTGGCCCTGCGCATTCCGGT
GATCGGCGACATTCTCGACAAGTCCTCGGTGGCGCGCTACTCGCGAACGCTTGCCACCACCTTCGGCGCCGGCGTGCCGC
TGGTGGAAGCGCTGGAGACCGCGCAGGGGGCGGCAGGCAACCTGGTGTATGAGCGCGCCATCGCCCAGATTCGCGAGGAC
GTGTCCAGCGGCCAGCAGCTCAACTTCGCCATGCGCATGACCGAAAAGTTCCCGGCGCTGGCGGTGCAGATGGTGGGCAT
CGGCGAGGAGGCCGGCTCGCTGGACGCCATGCTCAACCGGGTGGCGGACTACTACGAGGAGGAGGTCGACAACAAGGTGG
ATACCCTCACCTCGCTGCTCGAGCCCTTCATCATCGTGGTGCTGGGGGTGCTGGTCGGCGGCCTGGTGATCTCGATGTAC
CTGCCGATCTTCGAGCTGGGGAGCGTAATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(281-402)

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.208

98.537

0.534
  pilC Acinetobacter baylyi ADP1

52.393

96.829

0.507
  pilC Acinetobacter baumannii D1279779

50.882

96.829

0.493
  pilC Legionella pneumophila strain ERS1305867

48.878

97.805

0.478
  pilC Vibrio cholerae strain A1552

43.735

99.268

0.434
  pilC Vibrio campbellii strain DS40M4

43.25

97.561

0.422
  pilG Neisseria meningitidis 44/76-A

42.67

93.171

0.398
  pilG Neisseria gonorrhoeae MS11

42.67

93.171

0.398