Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P8934_RS10335 Genome accession   NZ_CP121193
Coordinates   2153480..2155276 (+) Length   598 a.a.
NCBI ID   WP_278030957.1    Uniprot ID   -
Organism   Halomonas sp. CKK8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2148480..2160276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8934_RS10295 (P8934_10295) - 2148779..2149378 (-) 600 WP_278030950.1 inorganic diphosphatase -
  P8934_RS10300 (P8934_10300) - 2149648..2150088 (-) 441 WP_347403435.1 phasin family protein -
  P8934_RS10305 (P8934_10305) - 2150385..2151092 (+) 708 WP_278030951.1 transposase -
  P8934_RS10310 (P8934_10310) - 2151230..2151529 (+) 300 WP_278030952.1 type II toxin-antitoxin system RelE/ParE family toxin -
  P8934_RS10315 (P8934_10315) - 2151526..2151822 (+) 297 WP_278030953.1 addiction module antidote protein -
  P8934_RS10320 (P8934_10320) - 2151897..2152163 (-) 267 WP_278030954.1 type II toxin-antitoxin system RelE/ParE family toxin -
  P8934_RS10325 (P8934_10325) - 2152147..2152368 (-) 222 WP_278030955.1 TraY domain-containing protein -
  P8934_RS10330 (P8934_10330) - 2152682..2153050 (-) 369 WP_278030956.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P8934_RS10335 (P8934_10335) pilB 2153480..2155276 (+) 1797 WP_278030957.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P8934_RS10340 (P8934_10340) pilC 2155565..2156797 (+) 1233 WP_278030958.1 type II secretion system F family protein Machinery gene
  P8934_RS10345 (P8934_10345) - 2156978..2157496 (-) 519 WP_278030959.1 L,D-transpeptidase -
  P8934_RS10350 (P8934_10350) - 2157496..2158164 (-) 669 WP_278030960.1 TetR/AcrR family transcriptional regulator -
  P8934_RS10355 (P8934_10355) lexA 2158602..2159255 (+) 654 WP_133633777.1 transcriptional repressor LexA -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 65108.70 Da        Isoelectric Point: 5.8088

>NTDB_id=810898 P8934_RS10335 WP_278030957.1 2153480..2155276(+) (pilB) [Halomonas sp. CKK8]
MNDYRPPSAESPPPAPEPSPSPLHGATQRGGLRGLARRLVADGLLGEAAAVRAESEAREAEMSLLEHLIEAGMVPARKAT
LTAAWEYGLPILDLDAIRLESLPPAADYPDKILRRLKVLPLVRHGHRLTVAVPYPSTLAMLDELQFATGLTPEGVLAPVD
QLGPVLETYLARNERSMLDELANVDDAVGELAYDEGVVDATDEQAAVAAAGDDAPIVKFVNKILLDAIHRGASDIHFEPY
ETSYRVRFRIDGILHDVAHPPFAMRSRISARLKVMSRLDISERRLPQDGAIKLKLSTTRSIDFRVNSLPTVYGEKVVLRI
LDPSSAQMGIEQLGFTPEQRAMYEAVLDDPQGMILVTGPTGSGKTVSLYTGINILNQVERNICTAEDPVEIKVPGVNQVN
VLPKIGLTFASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVAPFNIASSVSL
IIAQRLARRLCKRCKQPADIPREVLLQQGFTEEEVAGATIYEPVGCKHCTQGFKGRVGIYEVVPISDAMSHLIMTQGNSR
DFDTQARKEGHPDLRRSGLLKVMQGLTSLAEINRVTKD

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=810898 P8934_RS10335 WP_278030957.1 2153480..2155276(+) (pilB) [Halomonas sp. CKK8]
ATGAACGACTACCGTCCCCCCTCTGCCGAGAGCCCGCCGCCCGCGCCGGAGCCGTCGCCCTCGCCGCTGCATGGGGCCAC
CCAGCGCGGCGGGCTGCGCGGGCTGGCCCGGCGCCTGGTGGCCGATGGCCTGCTTGGCGAGGCGGCGGCGGTGCGTGCCG
AGAGCGAGGCCCGCGAGGCGGAGATGTCGCTGCTCGAGCATCTGATCGAGGCGGGCATGGTGCCGGCGCGCAAGGCCACG
CTCACCGCCGCCTGGGAGTATGGCCTGCCGATCCTCGACCTGGACGCCATCCGCCTGGAAAGCCTGCCGCCGGCGGCGGA
CTACCCGGACAAGATCCTGCGCCGGCTGAAGGTGCTGCCGCTGGTGCGCCACGGCCACCGGCTCACCGTGGCGGTGCCCT
ACCCCTCCACGCTGGCGATGCTCGACGAGCTGCAGTTCGCCACCGGCCTCACGCCGGAGGGGGTGCTGGCCCCGGTGGAC
CAGCTCGGCCCGGTGCTCGAGACCTACCTGGCGCGCAACGAGCGCAGCATGCTCGACGAGCTGGCCAACGTCGACGACGC
CGTGGGCGAGCTGGCCTATGACGAGGGCGTGGTGGACGCCACCGACGAGCAGGCGGCCGTGGCCGCGGCCGGTGACGACG
CGCCCATCGTCAAGTTCGTCAACAAGATCCTGCTGGATGCCATCCACCGCGGCGCCTCGGACATCCACTTCGAGCCGTAT
GAAACCAGCTACCGGGTGCGCTTTCGCATCGACGGCATCCTCCACGACGTGGCCCACCCGCCCTTCGCCATGCGCTCGCG
CATCTCGGCGCGCCTCAAGGTGATGTCGCGGCTGGATATCTCGGAGCGCCGCCTGCCCCAGGATGGCGCCATCAAGCTCA
AGCTCTCCACCACCCGCTCCATCGACTTTCGCGTCAACTCGCTGCCCACGGTGTACGGCGAGAAGGTAGTCCTGCGGATT
CTCGATCCCAGCTCGGCCCAGATGGGCATCGAGCAGCTGGGGTTCACCCCCGAGCAGCGCGCCATGTACGAGGCCGTGCT
CGACGACCCCCAGGGCATGATCCTGGTCACCGGGCCCACCGGCAGCGGCAAGACGGTCTCCCTCTATACCGGCATCAATA
TCCTCAACCAGGTGGAGCGCAACATCTGCACCGCCGAGGACCCGGTGGAGATCAAGGTGCCGGGGGTCAACCAGGTGAAC
GTGTTGCCCAAGATCGGCCTGACCTTCGCCAGCGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGGTGATGGTCGG
CGAGATCCGCGACCTGGAGACCGCCGAGATCGCCGTCAAGGCCTCGCAGACCGGCCACCTGGTGCTCTCCACCGTGCACA
CCAACTCGGCGGCGGAGACGCTGACGCGCCTGGCCAACATGGGCGTGGCGCCGTTCAATATCGCCAGCTCCGTGAGCCTG
ATCATCGCCCAGCGCCTGGCGCGGCGGCTCTGCAAGCGCTGCAAGCAGCCGGCGGACATCCCCCGGGAGGTGCTGCTGCA
GCAGGGCTTTACCGAGGAGGAGGTCGCCGGCGCCACCATCTATGAGCCGGTGGGCTGCAAGCACTGCACCCAGGGCTTCA
AGGGCCGGGTGGGCATCTACGAGGTGGTACCGATCAGTGATGCCATGAGCCACCTGATCATGACCCAGGGCAACTCCCGG
GACTTCGACACCCAGGCACGCAAGGAGGGCCACCCGGACCTGCGCCGCAGCGGGCTCTTGAAGGTGATGCAGGGACTGAC
CAGCCTGGCCGAGATCAACCGGGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(222-489)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(68-170)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.577

97.324

0.512
  pilB Legionella pneumophila strain ERS1305867

52.87

96.154

0.508
  pilB Acinetobacter baylyi ADP1

52.265

95.987

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

47.887

94.983

0.455
  pilB Vibrio cholerae strain A1552

49.907

89.799

0.448
  pilB Vibrio campbellii strain DS40M4

49.174

91.137

0.448
  pilF Neisseria gonorrhoeae MS11

45.87

95.151

0.436