Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P5S46_RS18395 Genome accession   NZ_CP120942
Coordinates   3966050..3967756 (+) Length   568 a.a.
NCBI ID   WP_277856344.1    Uniprot ID   A0AAJ5Z9J1
Organism   Aeromonas caviae strain AC1520     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3961050..3972756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5S46_RS18370 (P5S46_18370) pdhR 3962217..3962981 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  P5S46_RS18375 (P5S46_18375) ampD 3963258..3963830 (-) 573 WP_277856342.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  P5S46_RS18380 (P5S46_18380) - 3963959..3964429 (+) 471 WP_042015101.1 retropepsin-like aspartic protease -
  P5S46_RS18385 (P5S46_18385) nadC 3964433..3965296 (+) 864 WP_128341717.1 carboxylating nicotinate-nucleotide diphosphorylase -
  P5S46_RS18390 (P5S46_18390) - 3965607..3966050 (+) 444 WP_277856343.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P5S46_RS18395 (P5S46_18395) pilB 3966050..3967756 (+) 1707 WP_277856344.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  P5S46_RS18400 (P5S46_18400) pilC 3967884..3969125 (+) 1242 WP_277856345.1 type II secretion system F family protein Machinery gene
  P5S46_RS18405 (P5S46_18405) pilD 3969203..3970078 (+) 876 WP_277856346.1 A24 family peptidase Machinery gene
  P5S46_RS18410 (P5S46_18410) coaE 3970097..3970711 (+) 615 WP_042864857.1 dephospho-CoA kinase -
  P5S46_RS18415 (P5S46_18415) zapD 3970749..3971471 (+) 723 WP_010675825.1 cell division protein ZapD -
  P5S46_RS18420 (P5S46_18420) yacG 3971482..3971676 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  P5S46_RS18425 (P5S46_18425) mutT 3971734..3972138 (-) 405 WP_174213873.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62539.92 Da        Isoelectric Point: 5.8214

>NTDB_id=809482 P5S46_RS18395 WP_277856344.1 3966050..3967756(+) (pilB) [Aeromonas caviae strain AC1520]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
TQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=809482 P5S46_RS18395 WP_277856344.1 3966050..3967756(+) (pilB) [Aeromonas caviae strain AC1520]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTTG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTCGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCATACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGCATCAAGCTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGACTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCTTGGTGACCGGCCCTACCGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTGAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATCAACCTGCCCGGTGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAAGATCCCGACATCGTCATGGTAGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTTACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
ACGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAGAAGGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.026

99.824

0.549
  pilB Acinetobacter baylyi ADP1

55.062

99.12

0.546
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Vibrio cholerae strain A1552

54.093

98.944

0.535
  pilB Vibrio campbellii strain DS40M4

50.442

99.472

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.088

99.472

0.498
  pilF Neisseria gonorrhoeae MS11

46.561

99.824

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37