Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RMO93_RS20365 Genome accession   NZ_CP134805
Coordinates   4243162..4244547 (+) Length   461 a.a.
NCBI ID   WP_311819998.1    Uniprot ID   -
Organism   Escherichia coli strain E7-1N7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4238162..4249547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMO93_RS20340 (RMO93_20340) aroP 4238710..4240083 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  RMO93_RS20345 (RMO93_20345) ampE 4240126..4240980 (-) 855 WP_000172020.1 beta-lactamase regulator AmpE -
  RMO93_RS20350 (RMO93_20350) ampD 4240977..4241528 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RMO93_RS20355 (RMO93_20355) nadC 4241616..4242509 (+) 894 WP_137501332.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RMO93_RS20360 (RMO93_20360) pilA 4242712..4243152 (+) 441 WP_137504594.1 prepilin peptidase-dependent pilin Machinery gene
  RMO93_RS20365 (RMO93_20365) pilB 4243162..4244547 (+) 1386 WP_311819998.1 type II secretion system protein GspE Machinery gene
  RMO93_RS20370 (RMO93_20370) hofC 4244537..4245739 (+) 1203 WP_000157242.1 protein transport protein HofC -
  RMO93_RS20375 (RMO93_20375) guaC 4245774..4246817 (-) 1044 WP_001217338.1 GMP reductase -
  RMO93_RS20380 (RMO93_20380) - 4246973..4247017 (-) 45 WP_120795372.1 protein YacM -
  RMO93_RS20385 (RMO93_20385) coaE 4247042..4247662 (+) 621 WP_001269517.1 dephospho-CoA kinase -
  RMO93_RS20390 (RMO93_20390) zapD 4247662..4248405 (+) 744 WP_001194731.1 cell division protein ZapD -
  RMO93_RS20395 (RMO93_20395) yacG 4248415..4248612 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  RMO93_RS20400 (RMO93_20400) mutT 4248701..4249099 (-) 399 WP_000736040.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50553.21 Da        Isoelectric Point: 6.6254

>NTDB_id=808175 RMO93_RS20365 WP_311819998.1 4243162..4244547(+) (pilB) [Escherichia coli strain E7-1N7]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPGNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=808175 RMO93_RS20365 WP_311819998.1 4243162..4244547(+) (pilB) [Escherichia coli strain E7-1N7]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAGTCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAATCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACACTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGGCAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Vibrio campbellii strain DS40M4

48.837

83.948

0.41
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio cholerae strain A1552

46.891

83.731

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

46.753

83.514

0.39
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Haemophilus influenzae Rd KW20

44.703

83.948

0.375
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilF Thermus thermophilus HB27

40.587

88.72

0.36