Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RMO93_RS20360 Genome accession   NZ_CP134805
Coordinates   4242712..4243152 (+) Length   146 a.a.
NCBI ID   WP_137504594.1    Uniprot ID   -
Organism   Escherichia coli strain E7-1N7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4237712..4248152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMO93_RS20340 (RMO93_20340) aroP 4238710..4240083 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  RMO93_RS20345 (RMO93_20345) ampE 4240126..4240980 (-) 855 WP_000172020.1 beta-lactamase regulator AmpE -
  RMO93_RS20350 (RMO93_20350) ampD 4240977..4241528 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RMO93_RS20355 (RMO93_20355) nadC 4241616..4242509 (+) 894 WP_137501332.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RMO93_RS20360 (RMO93_20360) pilA 4242712..4243152 (+) 441 WP_137504594.1 prepilin peptidase-dependent pilin Machinery gene
  RMO93_RS20365 (RMO93_20365) pilB 4243162..4244547 (+) 1386 WP_311819998.1 type II secretion system protein GspE Machinery gene
  RMO93_RS20370 (RMO93_20370) hofC 4244537..4245739 (+) 1203 WP_000157242.1 protein transport protein HofC -
  RMO93_RS20375 (RMO93_20375) guaC 4245774..4246817 (-) 1044 WP_001217338.1 GMP reductase -
  RMO93_RS20380 (RMO93_20380) - 4246973..4247017 (-) 45 WP_120795372.1 protein YacM -
  RMO93_RS20385 (RMO93_20385) coaE 4247042..4247662 (+) 621 WP_001269517.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15650.80 Da        Isoelectric Point: 4.5237

>NTDB_id=808174 RMO93_RS20360 WP_137504594.1 4242712..4243152(+) (pilA) [Escherichia coli strain E7-1N7]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDTN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=808174 RMO93_RS20360 WP_137504594.1 4242712..4243152(+) (pilA) [Escherichia coli strain E7-1N7]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATAATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTTGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACACCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363