Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P5V12_RS20620 Genome accession   NZ_CP120416
Coordinates   4792673..4793815 (+) Length   380 a.a.
NCBI ID   WP_316954969.1    Uniprot ID   -
Organism   Teredinibacter sp. KSP-S5-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4787673..4798815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5V12_RS20590 (P5V12_20590) - 4787724..4788902 (-) 1179 WP_316954964.1 homoserine O-succinyltransferase MetX -
  P5V12_RS20595 (P5V12_20595) - 4788936..4789229 (-) 294 WP_316954965.1 DUF167 family protein -
  P5V12_RS20600 (P5V12_20600) - 4789233..4789823 (-) 591 WP_316954966.1 YggT family protein -
  P5V12_RS20605 (P5V12_20605) proC 4789832..4790611 (-) 780 WP_316957460.1 pyrroline-5-carboxylate reductase -
  P5V12_RS20610 (P5V12_20610) - 4790723..4791400 (-) 678 WP_316954967.1 YggS family pyridoxal phosphate-dependent enzyme -
  P5V12_RS20615 (P5V12_20615) pilT 4791490..4792524 (+) 1035 WP_316954968.1 type IV pilus twitching motility protein PilT Machinery gene
  P5V12_RS20620 (P5V12_20620) pilU 4792673..4793815 (+) 1143 WP_316954969.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P5V12_RS20625 (P5V12_20625) - 4794020..4794343 (+) 324 WP_316954970.1 hypothetical protein -
  P5V12_RS20630 (P5V12_20630) ruvX 4794356..4794790 (-) 435 WP_316954971.1 Holliday junction resolvase RuvX -
  P5V12_RS20635 (P5V12_20635) - 4794805..4795365 (-) 561 WP_316954972.1 YqgE/AlgH family protein -
  P5V12_RS20640 (P5V12_20640) - 4795523..4796398 (-) 876 WP_316954973.1 energy transducer TonB -
  P5V12_RS20645 (P5V12_20645) gshB 4796413..4797336 (-) 924 WP_316957461.1 glutathione synthase -
  P5V12_RS20650 (P5V12_20650) pilG 4797632..4798027 (+) 396 WP_316954974.1 twitching motility response regulator PilG Regulator
  P5V12_RS20655 (P5V12_20655) pilH 4798069..4798437 (+) 369 WP_316954975.1 twitching motility response regulator PilH -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42319.57 Da        Isoelectric Point: 5.9425

>NTDB_id=805532 P5V12_RS20620 WP_316954969.1 4792673..4793815(+) (pilU) [Teredinibacter sp. KSP-S5-2]
MDLDRLLNLMVEKKASDLFITAGVAPSIKLHGKIVPVTTTPLSPEKSREICLSVMNDKQRKEFMETKELNFAISARGVGR
FRASAFYQRNLAGMVLRRIETTIPKVDDLGLPEIIKELAMVKRGLVIFVGATGTGKSTSLASMIGHRNKNSKGHIISIED
PIEFVHQHDGCVITQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHSQLWMDLSLNLKAIVAQQLIPTPDGEGRRACLEVFLNTPLAADIIRKGAVHELKELMKKSTEQGMQTFDQA
LYELYDAGEITYEDALAHADSPNDLRLMIKLGSETDAEYLSNAADELSIQDEESNRTRLF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=805532 P5V12_RS20620 WP_316954969.1 4792673..4793815(+) (pilU) [Teredinibacter sp. KSP-S5-2]
ATGGATTTAGATCGTTTACTAAATTTAATGGTGGAAAAAAAGGCGTCCGACTTATTTATTACCGCGGGTGTCGCCCCTTC
AATTAAATTGCACGGAAAAATTGTTCCTGTCACGACTACGCCTTTGTCGCCGGAGAAATCACGTGAAATTTGTTTGAGTG
TGATGAACGATAAGCAGCGCAAAGAATTTATGGAAACCAAAGAGTTGAACTTTGCGATTAGTGCGCGAGGTGTGGGGCGT
TTCCGGGCGAGTGCGTTTTATCAGCGAAACCTGGCGGGAATGGTTTTGAGGCGTATCGAAACCACTATTCCGAAAGTGGA
TGACTTGGGATTGCCTGAGATTATTAAAGAGTTGGCAATGGTAAAGCGTGGTCTGGTGATATTTGTCGGGGCGACGGGTA
CTGGTAAATCAACCTCCCTGGCGTCGATGATTGGGCACCGAAACAAAAACTCCAAAGGCCATATTATTTCCATTGAAGAC
CCTATCGAATTTGTTCATCAGCACGACGGTTGTGTGATTACCCAGCGGGAAGTTGGTATTGATACCGAATCGTTTGAGGT
GGCTCTGAAGAATACTTTGCGGCAGGCTCCAGACGTAATTTTGATTGGTGAGGTGCGAACCAGAGAAACAATGGACCATG
CGATTGCTTTTGCTGAAACTGGTCACCTATGTTTATGTACGTTGCATGCTAACAATGCAAACCAGGCATTAGATCGTATT
ATCCACTTCTTCCCGGCAGATCGACATTCCCAATTGTGGATGGATTTATCTCTGAACCTGAAGGCTATTGTGGCGCAGCA
GCTTATTCCGACTCCAGATGGAGAAGGGCGACGAGCGTGTCTGGAGGTGTTTTTAAATACGCCGTTGGCGGCAGATATTA
TTCGAAAAGGCGCTGTACACGAGCTGAAAGAGTTGATGAAAAAATCCACTGAGCAGGGTATGCAGACTTTCGATCAGGCA
TTGTATGAATTATATGACGCTGGTGAAATTACCTACGAGGATGCACTAGCTCATGCTGACTCGCCAAACGATCTGCGCTT
GATGATTAAACTCGGTTCTGAAACCGATGCGGAATACCTCTCTAATGCGGCGGATGAATTAAGTATTCAGGACGAAGAAA
GTAATCGGACACGTCTATTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

75.526

100

0.755
  pilU Acinetobacter baylyi ADP1

67.507

93.947

0.634
  pilU Vibrio cholerae strain A1552

55.714

92.105

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.609

90.789

0.387
  pilT Legionella pneumophila strain Lp02

42.687

88.158

0.376
  pilT Legionella pneumophila strain ERS1305867

42.687

88.158

0.376