Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RJ646_RS19450 Genome accession   NZ_CP134358
Coordinates   4063152..4064537 (+) Length   461 a.a.
NCBI ID   WP_001025189.1    Uniprot ID   -
Organism   Escherichia coli strain TUM13936     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4058152..4069537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ646_RS19425 (RJ646_19425) - 4059136..4060086 (+) 951 WP_069906517.1 family 43 glycosylhydrolase -
  RJ646_RS19430 (RJ646_19430) ampE 4060116..4060970 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RJ646_RS19435 (RJ646_19435) ampD 4060967..4061518 (-) 552 WP_000923720.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RJ646_RS19440 (RJ646_19440) nadC 4061606..4062499 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RJ646_RS19445 (RJ646_19445) pilA 4062702..4063142 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  RJ646_RS19450 (RJ646_19450) pilB 4063152..4064537 (+) 1386 WP_001025189.1 type II secretion system protein GspE Machinery gene
  RJ646_RS19455 (RJ646_19455) hofC 4064527..4065729 (+) 1203 WP_276269955.1 protein transport protein HofC -
  RJ646_RS19460 (RJ646_19460) guaC 4065764..4066807 (-) 1044 WP_001217338.1 GMP reductase -
  RJ646_RS19465 (RJ646_19465) - 4066963..4067007 (-) 45 WP_120795372.1 protein YacM -
  RJ646_RS19470 (RJ646_19470) coaE 4067032..4067652 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  RJ646_RS19475 (RJ646_19475) zapD 4067652..4068395 (+) 744 WP_310984010.1 cell division protein ZapD -
  RJ646_RS19480 (RJ646_19480) yacG 4068405..4068602 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  RJ646_RS19485 (RJ646_19485) mutT 4068808..4069206 (-) 399 WP_000736023.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50634.31 Da        Isoelectric Point: 6.6268

>NTDB_id=802866 RJ646_RS19450 WP_001025189.1 4063152..4064537(+) (pilB) [Escherichia coli strain TUM13936]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=802866 RJ646_RS19450 WP_001025189.1 4063152..4064537(+) (pilB) [Escherichia coli strain TUM13936]
ATGAATATTCCACAGCTCACCGCCCTGTGTTTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAATTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CGCAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATTCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATTATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGCCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCATTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.385

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36