Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RJ646_RS19445 Genome accession   NZ_CP134358
Coordinates   4062702..4063142 (+) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain TUM13936     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4057702..4068142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ646_RS19420 (RJ646_19420) - 4057730..4059136 (+) 1407 WP_001088913.1 glycoside-pentoside-hexuronide (GPH):cation symporter -
  RJ646_RS19425 (RJ646_19425) - 4059136..4060086 (+) 951 WP_069906517.1 family 43 glycosylhydrolase -
  RJ646_RS19430 (RJ646_19430) ampE 4060116..4060970 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RJ646_RS19435 (RJ646_19435) ampD 4060967..4061518 (-) 552 WP_000923720.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RJ646_RS19440 (RJ646_19440) nadC 4061606..4062499 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RJ646_RS19445 (RJ646_19445) pilA 4062702..4063142 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  RJ646_RS19450 (RJ646_19450) pilB 4063152..4064537 (+) 1386 WP_001025189.1 type II secretion system protein GspE Machinery gene
  RJ646_RS19455 (RJ646_19455) hofC 4064527..4065729 (+) 1203 WP_276269955.1 protein transport protein HofC -
  RJ646_RS19460 (RJ646_19460) guaC 4065764..4066807 (-) 1044 WP_001217338.1 GMP reductase -
  RJ646_RS19465 (RJ646_19465) - 4066963..4067007 (-) 45 WP_120795372.1 protein YacM -
  RJ646_RS19470 (RJ646_19470) coaE 4067032..4067652 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=802865 RJ646_RS19445 WP_000360901.1 4062702..4063142(+) (pilA) [Escherichia coli strain TUM13936]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=802865 RJ646_RS19445 WP_000360901.1 4062702..4063142(+) (pilA) [Escherichia coli strain TUM13936]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363