Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PTW35_RS01980 Genome accession   NZ_CP119423
Coordinates   394595..396295 (-) Length   566 a.a.
NCBI ID   WP_281026344.1    Uniprot ID   -
Organism   Photobacterium sp. DA100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 389595..401295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTW35_RS01950 (PTW35_01950) mutT 390103..390501 (+) 399 WP_281026338.1 8-oxo-dGTP diphosphatase MutT -
  PTW35_RS01955 (PTW35_01955) yacG 390580..390798 (-) 219 WP_281026339.1 DNA gyrase inhibitor YacG -
  PTW35_RS01960 (PTW35_01960) zapD 390807..391544 (-) 738 WP_281026340.1 cell division protein ZapD -
  PTW35_RS01965 (PTW35_01965) coaE 391564..392184 (-) 621 WP_281026341.1 dephospho-CoA kinase -
  PTW35_RS01970 (PTW35_01970) pilD 392188..393057 (-) 870 WP_281026342.1 A24 family peptidase Machinery gene
  PTW35_RS01975 (PTW35_01975) pilC 393329..394549 (-) 1221 WP_281026343.1 type II secretion system F family protein Machinery gene
  PTW35_RS01980 (PTW35_01980) pilB 394595..396295 (-) 1701 WP_281026344.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PTW35_RS01985 (PTW35_01985) - 396295..396744 (-) 450 WP_281026345.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PTW35_RS01990 (PTW35_01990) nadC 397064..397951 (-) 888 WP_281027419.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PTW35_RS01995 (PTW35_01995) ampD 398489..399043 (+) 555 WP_039456936.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PTW35_RS02000 (PTW35_02000) pdhR 399358..400131 (+) 774 WP_281026346.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62623.74 Da        Isoelectric Point: 6.1351

>NTDB_id=799182 PTW35_RS01980 WP_281026344.1 394595..396295(-) (pilB) [Photobacterium sp. DA100]
MHTNLASILHQAELISPVQLQQVVDQVQAEKQSVPAALIGLGIMTSQDLARQLEVIFAVALVDAHQYDYAECCKTLNLRD
LILRHRVLPLTHTDNTLFIGLCDPTYLDALDDFRFATGKHVEPMLLEYKQLEAAIRRVYGSELKGTEQHAPQRGISETEL
TQLVELSDGELIDDTTDLSSDSAPVTRYINQTLLDALRKHASDIHFEPYEKHYRIRLRCDGILHQYATPPANLSRRLSTR
LKVMSRLNIAERRIPQDGRIKLKLSDTVSVDLRVSTLPTMWGEKIVLRILDSSSSKLDIDHLGYNEVQKQAYLDALNQPQ
GMILMTGPTGSGKTVSLYSGLKILNTEARNIATAEDPIEINLPGINQVQINPAAGLGFAEALRAFLRQDPDVVMVGEIRD
LETGGIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLSLIIAQRLARRLCSHCKQSDDLDPQLRARLEIP
LGAELFKASPNGCDDCNKGYLGRVGIYEVMPFSRPLSHALLQGASTLELEDIACQHGMITLRQAGIEKLCQGTTSLAELQ
RVLHFA

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=799182 PTW35_RS01980 WP_281026344.1 394595..396295(-) (pilB) [Photobacterium sp. DA100]
ATGCACACCAACCTCGCTTCAATCCTGCACCAGGCTGAGCTGATCAGCCCGGTGCAGTTGCAACAGGTGGTTGACCAGGT
CCAGGCTGAAAAGCAGTCGGTACCGGCGGCGTTGATTGGCCTTGGGATCATGACCAGCCAGGATCTGGCGCGCCAGCTGG
AGGTAATTTTTGCCGTTGCCTTGGTCGATGCCCACCAGTATGACTACGCCGAGTGCTGCAAGACCCTCAACCTGCGTGAT
TTGATCCTGCGCCACCGAGTACTGCCACTTACCCATACCGACAACACCCTGTTCATCGGCCTGTGCGACCCCACCTATCT
CGATGCCCTCGATGACTTCCGCTTTGCCACCGGCAAGCATGTCGAGCCGATGCTGCTGGAATACAAGCAGCTCGAGGCGG
CGATACGGCGGGTTTACGGCAGCGAGCTCAAAGGCACCGAACAGCACGCGCCGCAGCGCGGCATCAGCGAAACCGAGCTG
ACCCAACTGGTGGAGCTCAGCGATGGCGAGCTGATCGATGACACCACCGACCTCAGCAGCGACAGTGCCCCGGTCACCCG
CTACATCAACCAGACCCTACTCGATGCCCTGCGCAAGCACGCCTCGGATATCCATTTCGAGCCTTACGAGAAACACTACC
GGATCCGCTTGCGCTGCGACGGTATTTTGCACCAGTATGCCACCCCGCCAGCCAACCTCTCCCGCCGCCTGTCGACCCGG
CTAAAAGTCATGTCGCGGCTCAATATTGCCGAGCGGCGGATCCCGCAAGACGGCCGGATCAAGCTCAAACTGTCCGATAC
CGTCTCGGTGGATCTGCGGGTGTCGACCCTGCCGACCATGTGGGGCGAGAAAATCGTGTTGCGGATCCTCGATAGCAGCA
GTAGCAAGCTGGATATCGATCATCTCGGTTATAACGAGGTGCAAAAACAGGCCTACCTTGACGCGCTAAACCAGCCGCAG
GGCATGATCCTGATGACCGGGCCGACCGGAAGCGGCAAGACCGTCTCGCTCTATTCGGGACTGAAGATCCTCAATACCGA
AGCGCGCAACATCGCCACCGCCGAAGACCCGATTGAAATCAACCTGCCGGGGATCAACCAGGTCCAGATCAATCCCGCCG
CCGGCTTGGGCTTTGCCGAAGCACTGCGGGCTTTTCTACGCCAGGATCCGGACGTGGTGATGGTGGGCGAAATCCGCGAT
CTCGAAACCGGTGGCATTGCGGTCAAGGCCGCGCAGACCGGCCACCTGGTGCTATCGACCCTGCATACCAACTCGGCAGC
CGAAACCATCACCCGACTAACCAACATGGGGATCGAGGATTTCAATTTGGCCTCGTCACTCAGCCTGATCATCGCCCAGC
GCCTGGCCAGGCGGCTTTGCTCCCACTGCAAACAGTCGGACGATCTCGACCCGCAGCTCCGTGCCAGACTCGAGATCCCG
CTAGGTGCCGAGCTGTTCAAGGCCAGTCCGAACGGCTGTGATGACTGCAACAAGGGCTACCTGGGACGGGTCGGAATTTA
TGAAGTGATGCCCTTCTCAAGGCCGTTGTCACACGCCTTGTTGCAGGGAGCCAGTACCTTGGAGCTGGAGGATATCGCCT
GCCAGCACGGCATGATCACCCTCCGGCAAGCCGGTATCGAAAAGCTCTGCCAGGGCACCACCAGCCTCGCCGAGCTTCAG
CGTGTCCTGCATTTCGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.239

99.823

0.611
  pilB Vibrio parahaemolyticus RIMD 2210633

61.062

99.823

0.61
  pilB Vibrio campbellii strain DS40M4

60.354

99.823

0.602
  pilB Acinetobacter baylyi ADP1

49.548

97.703

0.484
  pilB Acinetobacter baumannii D1279779

49.097

97.88

0.481
  pilB Legionella pneumophila strain ERS1305867

49.168

95.583

0.47
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

37.521

100

0.396
  pilF Thermus thermophilus HB27

37.986

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.255

99.117

0.369