NTDB

Overview

Name	pilQ	Type	Machinery gene
Locus tag	PTW35_RS01045	Genome accession	NZ_CP119423
Coordinates	215173..216924 (+)	Length	583 a.a.
NCBI ID	WP_281026194.1	Uniprot ID	-
Organism	Photobacterium sp. DA100
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	214041..241864	215173..216924	within	0

Gene organization within MGE regions

Location: 214041..241864

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PTW35_RS01035 (PTW35_01035)	pilO	214041..214631 (+)	591	WP_281026191.1	type 4a pilus biogenesis protein PilO	-
PTW35_RS01040 (PTW35_01040)	-	214628..215161 (+)	534	WP_281026193.1	pilus assembly protein PilP	-
PTW35_RS01045 (PTW35_01045)	pilQ	215173..216924 (+)	1752	WP_281026194.1	type IV pilus secretin PilQ family protein	Machinery gene
PTW35_RS01050 (PTW35_01050)	aroK	217138..217656 (+)	519	WP_281026195.1	shikimate kinase AroK	-
PTW35_RS01055 (PTW35_01055)	aroB	217675..218754 (+)	1080	WP_281026196.1	3-dehydroquinate synthase	-
PTW35_RS01060 (PTW35_01060)	-	218770..220350 (+)	1581	WP_281026197.1	AAA family ATPase	-
PTW35_RS01065 (PTW35_01065)	dam	220455..221279 (+)	825	WP_281026198.1	adenine-specific DNA-methyltransferase	-
PTW35_RS01070 (PTW35_01070)	-	221287..221484 (-)	198	WP_281026199.1	DUF2970 domain-containing protein	-
PTW35_RS01075 (PTW35_01075)	rpe	221678..222352 (+)	675	WP_039469006.1	ribulose-phosphate 3-epimerase	-
PTW35_RS01080 (PTW35_01080)	-	222352..223041 (+)	690	WP_281026200.1	phosphoglycolate phosphatase	-
PTW35_RS01085 (PTW35_01085)	trpS	223128..224144 (+)	1017	WP_039469001.1	tryptophan--tRNA ligase	-
PTW35_RS01090 (PTW35_01090)	-	224266..224841 (+)	576	WP_039468998.1	aminodeoxychorismate/anthranilate synthase component II	-
PTW35_RS01095 (PTW35_01095)	-	225246..226460 (+)	1215	WP_281026201.1	aspartate aminotransferase family protein	-
PTW35_RS01100 (PTW35_01100)	astA	226571..227581 (+)	1011	WP_281026202.1	arginine N-succinyltransferase	-
PTW35_RS01105 (PTW35_01105)	astD	227593..229050 (+)	1458	WP_281026203.1	succinylglutamate-semialdehyde dehydrogenase	-
PTW35_RS01110 (PTW35_01110)	-	229095..229892 (+)	798	WP_281026204.1	DUF1338 domain-containing protein	-
PTW35_RS01115 (PTW35_01115)	-	229986..230174 (-)	189	WP_281026205.1	hypothetical protein	-
PTW35_RS01120 (PTW35_01120)	crp	230362..230994 (-)	633	WP_036801200.1	cAMP-activated global transcriptional regulator CRP	-
PTW35_RS01125 (PTW35_01125)	-	231265..231672 (+)	408	WP_281026207.1	OsmC family protein	-
PTW35_RS01130 (PTW35_01130)	-	231683..232261 (-)	579	WP_281026208.1	DUF3501 family protein	-
PTW35_RS01135 (PTW35_01135)	-	232285..233613 (-)	1329	WP_281026209.1	heterodisulfide reductase-related iron-sulfur binding cluster	-
PTW35_RS01140 (PTW35_01140)	-	233678..234097 (-)	420	WP_281026210.1	rubrerythrin family protein	-
PTW35_RS01145 (PTW35_01145)	-	234211..235080 (-)	870	WP_281026211.1	phosphoribulokinase	-
PTW35_RS01150 (PTW35_01150)	-	235186..235407 (-)	222	WP_281026212.1	YheU family protein	-
PTW35_RS01155 (PTW35_01155)	-	235638..236618 (-)	981	WP_281026213.1	hydrolase	-
PTW35_RS01160 (PTW35_01160)	-	236634..237092 (-)	459	WP_281026214.1	TIGR02444 family protein	-
PTW35_RS01165 (PTW35_01165)	-	237079..239001 (-)	1923	WP_044622381.1	ABC transporter ATP-binding protein	-
PTW35_RS01170 (PTW35_01170)	kefG	239241..239810 (+)	570	WP_281026215.1	glutathione-regulated potassium-efflux system ancillary protein KefG	-
PTW35_RS01175 (PTW35_01175)	kefB	239800..241611 (+)	1812	WP_281026216.1	glutathione-regulated potassium-efflux system protein KefB	-
PTW35_RS01180 (PTW35_01180)	-	241652..241864 (+)	213	WP_281026217.1	YheV family putative zinc ribbon protein	-

Sequence

Protein

Download Length: 583 a.a. Molecular weight: 62789.95 Da Isoelectric Point: 4.8012

                                >NTDB_id=799177 PTW35_RS01045 WP_281026194.1 215173..216924(+) (pilQ) [Photobacterium sp. DA100]

MTRKWLQALRLRWAKRVARGGWGVLLGVALLHGHAVAANAVTQISSIDFSRNVKGDGVLEVVLSSGAVEVGARRLDKQLV

IELPQAELPEELVYVLDVKEFGTPVLAVETFQVTSGSRLVLAVEGDYRYDYITRDTAGSTSLLVTVQPAVQPQGGNGGGA

GIRYDSKPISINFQDVPVRNVLQLIAEYNDFNLVVSDSVQGNLTLRLDEVPWQQVLDIILRAKGLDKREQGSVLMVAPKE

ELARSEQQVLESKRKAEELASLRSEIFKINYANALELGGLLQGADDGISMLSARGSLHIDERTNSLIINDIPDSLANIRD

IVAALDIPVQQVEIEARIVTINEGDIDELGVRWGILKTNGSSTVGGSIEGNLASAGLLDITDGEKAVENFLNVNLGATSP

GASSIAFQVAKMGDILLDLELSALQSEQKAEVISSPRLVTTNKKMAYIEQGTEIPYLEAASSGAVSVSFKKAVLSLMVTP

QITSDGNLVLDLVVTQDKKGKVVQTGTGEAVAIDTQRIGTQVLVANGETVVLGGIYQHAINNTVRKVPLLGDLPMLGALF

RHRLEELGKKELLIFVTPKIVYQ

Nucleotide

Download Length: 1752 bp

                                >NTDB_id=799177 PTW35_RS01045 WP_281026194.1 215173..216924(+) (pilQ) [Photobacterium sp. DA100]

ATGACAAGGAAATGGTTGCAGGCCCTGCGGTTGAGATGGGCAAAGAGAGTGGCACGGGGTGGCTGGGGGGTACTGCTCGG

CGTGGCGTTGCTGCATGGCCATGCGGTTGCCGCCAACGCGGTCACGCAGATCAGCAGTATCGACTTTTCCCGTAACGTAA

AGGGGGACGGCGTGTTGGAGGTGGTGTTGTCGAGCGGGGCCGTTGAGGTCGGTGCTCGGCGGCTGGATAAGCAACTGGTG

ATCGAGTTGCCGCAGGCAGAGCTGCCCGAGGAGTTGGTCTATGTGCTTGATGTCAAGGAGTTCGGCACCCCGGTACTCGC

CGTTGAAACTTTCCAGGTGACAAGCGGTAGCCGGTTGGTACTGGCCGTTGAGGGGGATTACCGCTACGACTACATCACCC

GGGATACGGCAGGGAGCACGTCGCTGTTAGTGACGGTACAGCCGGCCGTGCAGCCGCAGGGGGGCAATGGTGGCGGAGCG

GGGATCCGCTACGATAGCAAGCCGATTTCGATTAACTTCCAAGATGTGCCGGTACGCAACGTGCTGCAACTTATCGCCGA

GTATAACGATTTCAACTTGGTGGTCTCAGACTCGGTGCAGGGTAACCTGACTTTACGCCTCGACGAGGTACCCTGGCAAC

AGGTACTGGATATCATTCTCCGGGCTAAGGGACTGGACAAGCGAGAGCAGGGCTCGGTATTGATGGTAGCACCCAAGGAG

GAGCTCGCCCGCAGCGAGCAGCAGGTGCTGGAATCGAAGCGCAAGGCCGAGGAGCTGGCCTCGCTGCGTTCGGAGATCTT

CAAGATAAATTACGCCAATGCGCTTGAACTGGGAGGGCTGCTGCAGGGGGCCGATGACGGGATCAGCATGCTATCGGCCC

GGGGCTCACTGCATATCGATGAGCGGACCAACTCTCTGATCATCAATGATATTCCTGACAGCCTGGCCAATATCCGTGAC

ATAGTGGCAGCGCTAGACATACCGGTACAGCAGGTCGAAATCGAAGCCAGGATTGTCACCATCAACGAGGGGGATATCGA

TGAACTGGGCGTCCGTTGGGGGATCCTCAAGACCAACGGCAGCTCGACGGTTGGTGGCTCGATCGAGGGGAATTTGGCGT

CGGCCGGGCTGCTGGATATCACCGATGGCGAGAAGGCGGTGGAGAACTTCCTCAATGTCAACTTGGGGGCAACCTCTCCC

GGCGCCTCAAGCATTGCCTTTCAGGTCGCCAAGATGGGCGATATCTTGCTCGACCTGGAGCTCTCGGCCCTGCAGTCGGA

ACAGAAGGCTGAAGTGATCTCCAGCCCAAGGCTGGTGACCACCAACAAGAAAATGGCCTACATCGAGCAGGGAACTGAAA

TTCCCTACCTCGAGGCGGCTTCCAGCGGTGCCGTTTCGGTCAGCTTCAAGAAAGCGGTGCTGAGCCTGATGGTGACCCCG

CAAATCACCTCGGACGGTAACCTGGTGCTGGATCTGGTGGTGACCCAGGACAAAAAAGGCAAGGTGGTGCAAACCGGTAC

GGGTGAAGCGGTGGCCATCGACACCCAGCGTATCGGTACCCAGGTGCTGGTGGCAAACGGCGAAACGGTGGTGCTCGGCG

GGATCTACCAGCACGCCATCAATAATACGGTACGCAAGGTGCCGCTGCTGGGGGATTTGCCCATGCTGGGGGCACTGTTC

CGCCATCGGCTGGAAGAGCTGGGCAAGAAGGAGCTGCTGATTTTTGTCACGCCCAAAATTGTCTACCAGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(265-331)

Secretin and TonB N terminus short domain (STN)

(191-239)

Bacterial type II and III secretion system protein (Secretin)

(423-581)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilQ	Vibrio campbellii strain DS40M4	60.862	95.54	0.581
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	59.019	97.942	0.578
pilQ	Vibrio cholerae strain A1552	59.019	97.942	0.578
comQ	Acinetobacter baylyi ADP1	39.619	99.142	0.393
pilQ	Legionella pneumophila strain ERS1305867	40.472	94.511	0.383
pilQ	Legionella pneumophila strain Lp02	40.472	94.511	0.383
pilQ	Pseudomonas aeruginosa PAK	39.64	95.197	0.377
pilQ	Acinetobacter baumannii D1279779	37.177	99.657	0.37
pilQ	Acinetobacter baumannii strain A118	37.177	99.657	0.37

Detailed information NTG0799177