Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PY372_RS12885 Genome accession   NZ_CP119301
Coordinates   2767534..2767938 (+) Length   134 a.a.
NCBI ID   WP_015297250.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain LC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2762534..2772938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY372_RS12870 (PY372_13045) pdhR 2764567..2765334 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PY372_RS12875 (PY372_13050) ampD 2765740..2766291 (-) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PY372_RS12880 (PY372_13055) nadC 2766384..2767271 (+) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PY372_RS12885 (PY372_13060) pilA 2767534..2767938 (+) 405 WP_015297250.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  PY372_RS12890 (PY372_13065) pilB 2767938..2769623 (+) 1686 WP_053339791.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PY372_RS12895 (PY372_13070) pilC 2769647..2770870 (+) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  PY372_RS12900 (PY372_13075) pilD 2770935..2771804 (+) 870 WP_025552578.1 prepilin peptidase Machinery gene
  PY372_RS12905 (PY372_13080) coaE 2771805..2772419 (+) 615 WP_005480887.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13659.55 Da        Isoelectric Point: 6.4433

>NTDB_id=798395 PY372_RS12885 WP_015297250.1 2767534..2767938(+) (pilA) [Vibrio parahaemolyticus strain LC]
MKHSKQKKQQGFTLIELMIVVAIIGVLSAIAVPAYQNYVAKSEAATALGSLRALVTPAELKLQQDGELSGVVADLGGSAS
HALGAITTSGANISAATLTFTFNTGSLSGDAITLTKTSSGWTCTDGTTVLDNCN

Nucleotide


Download         Length: 405 bp        

>NTDB_id=798395 PY372_RS12885 WP_015297250.1 2767534..2767938(+) (pilA) [Vibrio parahaemolyticus strain LC]
ATGAAACACAGTAAACAAAAAAAACAACAGGGTTTTACTCTGATCGAATTAATGATTGTGGTGGCGATTATTGGGGTGTT
GTCAGCAATTGCAGTACCTGCTTATCAAAATTATGTGGCTAAAAGTGAAGCGGCAACGGCATTAGGAAGTTTGAGAGCAT
TAGTCACTCCTGCTGAACTTAAACTTCAACAAGATGGAGAGCTATCAGGTGTAGTAGCTGACTTAGGTGGATCTGCATCA
CATGCATTAGGTGCTATTACAACATCTGGTGCTAACATAAGCGCTGCTACTCTAACCTTCACGTTTAATACAGGCTCATT
AAGTGGTGATGCTATCACACTAACCAAAACAAGTTCTGGTTGGACATGTACTGATGGCACTACGGTTTTAGACAACTGTA
ACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-130)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.143

100

0.545
  pilA Pseudomonas aeruginosa PAK

42.446

100

0.44
  pilA Vibrio cholerae strain A1552

42.029

100

0.433
  pilA Vibrio cholerae C6706

42.029

100

0.433
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.029

100

0.433
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.188

95.522

0.403
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.535

96.269

0.381