Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PYU98_RS02050 Genome accession   NZ_CP118988
Coordinates   405661..407367 (-) Length   568 a.a.
NCBI ID   WP_042058702.1    Uniprot ID   A0A6S5YZF1
Organism   Aeromonas allosaccharophila strain K520     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 400661..412367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYU98_RS02020 (PYU98_02020) mutT 401254..401658 (+) 405 WP_270794725.1 8-oxo-dGTP diphosphatase MutT -
  PYU98_RS02025 (PYU98_02025) yacG 401736..401936 (-) 201 WP_213392112.1 DNA gyrase inhibitor YacG -
  PYU98_RS02030 (PYU98_02030) zapD 401943..402665 (-) 723 WP_275057314.1 cell division protein ZapD -
  PYU98_RS02035 (PYU98_02035) coaE 402703..403317 (-) 615 WP_275057315.1 dephospho-CoA kinase -
  PYU98_RS02040 (PYU98_02040) pilD 403331..404203 (-) 873 WP_275057316.1 A24 family peptidase Machinery gene
  PYU98_RS02045 (PYU98_02045) pilC 404265..405506 (-) 1242 WP_234605458.1 type II secretion system F family protein Machinery gene
  PYU98_RS02050 (PYU98_02050) pilB 405661..407367 (-) 1707 WP_042058702.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PYU98_RS02055 (PYU98_02055) - 407371..407808 (-) 438 WP_275057317.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PYU98_RS02060 (PYU98_02060) nadC 408124..408987 (-) 864 WP_275057318.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PYU98_RS02065 (PYU98_02065) - 408991..409461 (-) 471 WP_275057319.1 retropepsin-like aspartic protease -
  PYU98_RS02070 (PYU98_02070) ampD 409592..410167 (+) 576 WP_275057320.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PYU98_RS02075 (PYU98_02075) pdhR 410446..411210 (+) 765 WP_275057321.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62477.87 Da        Isoelectric Point: 5.4321

>NTDB_id=797164 PYU98_RS02050 WP_042058702.1 405661..407367(-) (pilB) [Aeromonas allosaccharophila strain K520]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENSILDSKALADFCELEYGVPLLDLAAFDLAEIPQKF
LNQKLIDKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIAKLLENESDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=797164 PYU98_RS02050 WP_042058702.1 405661..407367(-) (pilB) [Aeromonas allosaccharophila strain K520]
ATGACTTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCTCAGCGCTA
TCTGAGCCAAGCTAAGGCCCAGCGAAAACCCTTTGTGACCTTTCTGATCGAGAACAGCATCCTCGACAGCAAGGCGCTGG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTTC
CTCAATCAAAAGCTGATCGACAAGCACCACGTTCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCGCTGGAGGACTTCGGCTTCAGCTTCGGGCTCCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATAGCCAAGCTGCTGGAGAACGAGAGCGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGTCGGCTGGACGAGAGCGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCCTCCGACCTGCACTTCGAACCCTACGAGTTCAAGTATC
GGGTCCGCTTTCGCATCGACGGCATCCTGCACGAGATCGCCACCCCGCCGGTCAATCTGGCCAACCGCTTCTCCGCCCGT
CTCAAGGTAATGGCACGGCTCGATATCGCCGAGCGGCGGCTACCACAGGATGGCCGCATCAAGCTGAAGCTGTCGCGTAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTGATCCGTCTGCTCGACTCCTCCG
CTGCGCGACTCAATATCGAGCAGCTTGGCTTTGATGACCGGCAGAAGGCGCAATACCTGCGCGCTCTCTCCAAGCCGCAG
GGGATGATCCTGGTGACAGGCCCCACCGGCTCGGGCAAAACTGTCTCGCTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTGAACATCTCCACCGCCGAAGATCCGGTAGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGATCCGGATGTGGTGATGGTGGGAGAGATCCGTGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCGGCCCAAACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCAGCCGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTGCCGGCCTTCAATATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAGGTGGTGCCGGAAGCCGAACTACTGGAACTGGGCTTTACC
CCGCAGCAGCTGGCGGCCGGATTGCGGTTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCGCTGCAAATCGCCG
CAATTGCCCAGAAAGAGGGGATGCGTACCCTGCGTACCTCAGGGCTGGAGAAGGCCCGACTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5YZF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.458

95.423

0.539
  pilB Vibrio cholerae strain A1552

54.578

98.063

0.535
  pilB Vibrio campbellii strain DS40M4

50.523

100

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

51.241

99.296

0.509
  pilF Neisseria gonorrhoeae MS11

47.163

99.296

0.468
  pilF Thermus thermophilus HB27

40.53

92.958

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

38.156

97.359

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361