Detailed information NTG0796958

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: PYW37_RS06190
• Genome accession: NZ_CP118969
• Coordinates: 1228144..1228992 (+)
• Length: 282 a.a.
• NCBI ID: WP_012897825.1
• Uniprot ID: A0A2A9I6Y4
• Organism: Lactococcus lactis strain DSM 20481
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1223144..1233992

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PYW37_RS06165	ilvN	1223902..1224378 (+)	477	WP_010905839.1	acetolactate synthase small subunit	-
PYW37_RS06170	ilvC	1224424..1225446 (+)	1023	WP_025017225.1	ketol-acid reductoisomerase	-
PYW37_RS06175	ilvA	1225502..1226752 (+)	1251	WP_017864495.1	threonine ammonia-lyase IlvA	-
PYW37_RS06180	budA	1226868..1227577 (+)	710	Protein_1209	acetolactate decarboxylase	-
PYW37_RS06185	-	1227618..1227998 (+)	381	WP_003131142.1	RidA family protein	-
PYW37_RS06190	dprA	1228144..1228992 (+)	849	WP_012897825.1	DNA-processing protein DprA	Machinery gene
PYW37_RS06195	topA	1229254..1231386 (+)	2133	WP_023189256.1	type I DNA topoisomerase	-
PYW37_RS06200	trmFO	1231458..1232801 (+)	1344	WP_025017071.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
PYW37_RS06205	xerS	1232846..1233916 (-)	1071	WP_003131037.1	tyrosine recombinase XerS	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 32119.17 Da
• Isoelectric Point: 9.5086

>NTDB_id=796958 PYW37_RS06190 WP_012897825.1 1228144..1228992(+) (dprA) [Lactococcus lactis strain DSM 20481]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDVSKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide

• Length: 849 bp

>NTDB_id=796958 PYW37_RS06190 WP_012897825.1 1228144..1228992(+) (dprA) [Lactococcus lactis strain DSM 20481]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGACGTAAGTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGGCTCAAGGAAATTTACAATCCACCGGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAGTATCTTGCTAAAAACCA
ACTCATACTATCAGAATATTCAGTCGGAGAAAAGCCACTTCGTTATCATTTTCCTGAACGTAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATACAACAAGGAGCAAA
ATTAATCTATCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

• DNA recombination-mediator protein A (DNA_processg_A) (76-270)

3D structure

• AlphaFold DB: A0A2A9I6Y4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	86.879	100	0.869
dprA	Streptococcus mutans UA159	60.072	98.582	0.592
dprA/cilB/dalA	Streptococcus mitis SK321	54.643	99.291	0.543
dprA/cilB/dalA	Streptococcus pneumoniae R6	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae D39	53.929	99.291	0.535
dprA	Legionella pneumophila strain ERS1305867	39.13	97.872	0.383
dprA	Haemophilus influenzae Rd KW20	38.889	95.745	0.372
dprA	Bacillus subtilis subsp. subtilis str. 168	43.777	82.624	0.362
dprA	Latilactobacillus sakei subsp. sakei 23K	40	90.426	0.362