Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PYV58_RS20430 Genome accession   NZ_CP118892
Coordinates   4363557..4365269 (+) Length   570 a.a.
NCBI ID   WP_275059652.1    Uniprot ID   A0AAX3PJY0
Organism   Acinetobacter sp. TAC-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4358557..4370269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYV58_RS20390 (PYV58_20390) nusA 4359300..4360784 (-) 1485 WP_004718337.1 transcription termination factor NusA -
  PYV58_RS20395 (PYV58_20395) rimP 4360819..4361343 (-) 525 WP_004818614.1 ribosome maturation factor RimP -
  PYV58_RS20420 (PYV58_20420) secG 4362134..4362475 (-) 342 WP_034613616.1 preprotein translocase subunit SecG -
  PYV58_RS20425 (PYV58_20425) tpiA 4362485..4363279 (-) 795 WP_034613615.1 triose-phosphate isomerase -
  PYV58_RS20430 (PYV58_20430) pilB 4363557..4365269 (+) 1713 WP_275059652.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PYV58_RS20435 (PYV58_20435) pilC 4365297..4366526 (+) 1230 WP_034613611.1 type II secretion system F family protein Machinery gene
  PYV58_RS20440 (PYV58_20440) pilD 4366532..4367413 (+) 882 WP_171077762.1 A24 family peptidase Machinery gene
  PYV58_RS20445 (PYV58_20445) coaE 4367440..4368039 (+) 600 WP_034613606.1 dephospho-CoA kinase -
  PYV58_RS20450 (PYV58_20450) - 4368108..4369019 (-) 912 WP_034613603.1 DMT family transporter -
  PYV58_RS20455 (PYV58_20455) rlmB 4369058..4369813 (-) 756 WP_004818598.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63457.93 Da        Isoelectric Point: 5.0224

>NTDB_id=796115 PYV58_RS20430 WP_275059652.1 4363557..4365269(+) (pilB) [Acinetobacter sp. TAC-1]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALSNAQKNKVSIVAYLSEKLNISPLIIAEMISEEFGEPIFDLDSFNPDML
PKELIDSNLVSKHKVIPLLQRGNILYVATSDPTNIEALDAIRFNCGLNIEPIIVEHHKLEKYLEKHFTVEESFNFGDEDF
DVDIIATDDNEPTNIDEDTAEDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLASRLAS
RLKVMSRMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEKQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLSFSVALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPVEIPRQSLLEMGF
TEQDLSNPDLQIFRPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLIKVINGLTS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=796115 PYV58_RS20430 WP_275059652.1 4363557..4365269(+) (pilB) [Acinetobacter sp. TAC-1]
ATGTCTATGCAAAATGGTTCACCTAAGTTCTCGGGGTATATCCGTCGCTTAGTCGACGAAGGTTTTTTATCTGCCCAGCA
AATGAAAGATGCGCTGAGTAATGCTCAAAAAAATAAAGTGAGTATTGTCGCTTATCTTAGTGAGAAATTAAATATATCGC
CACTGATCATTGCTGAAATGATCTCCGAAGAATTTGGAGAACCTATTTTTGATCTGGACAGCTTTAATCCTGACATGCTG
CCCAAGGAGCTTATTGATTCAAATTTAGTTAGCAAACATAAAGTGATTCCTTTGCTGCAACGTGGCAATATTTTATATGT
CGCCACCAGTGATCCGACCAATATAGAGGCGCTGGATGCCATTCGTTTTAACTGCGGTTTGAATATTGAACCAATCATTG
TTGAACATCATAAATTAGAAAAATATTTAGAAAAGCACTTTACGGTAGAAGAAAGTTTTAATTTTGGTGATGAAGATTTT
GATGTTGATATTATTGCAACTGATGATAACGAACCAACAAATATTGATGAAGATACTGCTGAAGATGAAGCACCTATTGT
AAAATATATCAATAAACTCTTGGTCGATGCGATTCGTATGGGAGCATCAGATTTACACTTTGAACCTTATGAAAAAAGTT
ATCGGGTTCGTTATCGTGTAGATGGGGTACTGAGAACCATTACCAATCCCCCCTTACAATTAGCAAGTCGTTTGGCCTCG
CGCTTAAAAGTGATGTCCAGAATGGACATTTCAGAAAAACGTGTTCCGCAAGATGGTCGCATTAAGTTAAAACTCTCAAA
AACTAAAGCTATTGATTTTCGTGTGAACTCACTGCCGACATTATTTGGCGAAAAATTGGTACTGCGTATTCTTGATCCTT
CCAGTGCCATGTTGGGTATTGATGCGTTAGGTTATGAAGAAAAACAAAAAGCATTATTTATGGAGGCCTTAGAAAAACCT
CAAGGCATGTTACTGATTACAGGACCCACAGGTTCAGGTAAAACTGTTTCCCTCTATACAGGTTTAAATATCCTCAATAC
AGAAAGTTCAAATATTTCCACGGCTGAAGATCCAGTTGAAATTAATTTGGAAGGGATTAATCAGGTTAACGTTAATCTTA
AAGCAGGACTTAGCTTTTCGGTCGCTTTGAAATCATTCCTACGTCAAGATCCAGACATTATCATGGTCGGGGAGATCCGA
GACCTTGAAACTGCTGAAATTGCGATTAAAGCTGCTCAAACGGGTCATATGGTGATGTCTACCTTACATACCAACAGTGC
ACCTGAAACACTGACCCGTTTACGTAATATGGGTGTGCCTTCATTTAACATTGCCACTTCAGTCAATCTTGTGATTGCGC
AACGATTGGCAAGACGTTTATGTAATCATTGTAAAGCACCTGTTGAAATCCCTAGACAGAGTCTGTTAGAAATGGGTTTT
ACTGAACAAGATTTAAGCAATCCCGATTTACAAATTTTCCGACCCGTAGGATGTAGTGAATGTCGTGAAGGCTATAAAGG
TCGTGTCGGGATCTATGAAGTGATGAAAGTAACACCTGAAATTTCAAAAATTATCATGGAAGATGGCAATGCACTTCAAA
TTGCAGCTGCATCGGAGAAATTGGGGTTTGATAATCTAAGACGTTCGGGTTTAATTAAGGTCATCAATGGTTTAACCTCT
TTACAAGAGATTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.772

100

0.788
  pilB Acinetobacter baylyi ADP1

74.912

100

0.749
  pilB Legionella pneumophila strain ERS1305867

51.13

100

0.516
  pilF Neisseria gonorrhoeae MS11

50.089

98.772

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

48.746

97.895

0.477
  pilB Vibrio cholerae strain A1552

49.002

96.667

0.474
  pilB Vibrio campbellii strain DS40M4

47.67

97.895

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

34.578

100

0.374
  pilF Thermus thermophilus HB27

36.14

100

0.361